- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB3238,Anti-Cytokeratin 19 Antibody, clone RCK108
产品名称：Anti-Cytokeratin 19 Antibody, clone RCK108
产品型号：MAB3238
Anti-Cytokeratin 19 Antibody, clone RCK108 is an antibody against Cytokeratin 19 for use in FC, WB, IC, IH(P).
merck millipore,默克密理博,MAB3238,Anti-Cytokeratin 19 Antibody, clone RCK108
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3238 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Cytokeratin 19 Antibody, clone RCK108 背景信息 Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.产品信息 格式 Purified 演示 Liquid in buffer with 0.1% sodium azide. 应用 应用 Anti-Cytokeratin 19 Antibody, clone RCK108 is an antibody against Cytokeratin 19 for use in FC, WB, IC, IH(P). 主要应用 - Immunocytochemistry
- Flow Cytometry
- Western Blotting
应用说明 Western blot: 1:100-1:1,000; Recognizes a 40kDa band in reduced westerns
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Cytoskeletal preparation of T24 cells. 克隆 RCK108 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Cytokeratin 19. MAB3238 reacts with glandular epithelial cells and most carcinomas. Does not react with hepatocytes and hepatocarcinoma 同种型 IgG1κ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002276.4
Entrez基因汇总 The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. 基因符号 - CK-19
- KRT19
- Keratin-19
- K19
- CK19
- MGC15366
- K1CS
- Cytokeratin-19
UniProt编号 - P08727
UniProt汇总 FUNCTION: SwissProt: P08727 # Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
SIZE: 400 amino acids; 44092 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.
TISSUE SPECIFICITY: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.DEVELOPMENTAL STAGE: Present in hair follicles at all stages of development.
DOMAIN: SwissProt: P08727 This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,04-964,Anti-Interleukin 4 (IL-4) Antibody, clone 11.B.11
下一件merck millipore产品：merck millipore,默克密理博,48-612MAG,MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
相关产品
merck millipore,默克密理博,07-1534,Anti-JMJD3 Antibody (CT)
merck millipore,默克密理博,MABT67,Anti-L-Selectin (CD62L) Antibody, clone MEL-14
merck millipore,默克密理博,06-1037,Anti-TMEM126A Antibody
merck millipore,默克密理博,444247,MMP Inhibitor II - CAS 203915-59-7 - Calbiochem
merck millipore,默克密理博,04-964,Anti-Interleukin 4 (IL-4) Antibody, clone 11.B.11
merck millipore,默克密理博,PP100,IgG1 Antibody, Mouse
merck millipore,默克密理博,ABS615,Anti-PHIP Antibody
merck millipore,默克密理博,ABC286,Anti-CTSL2 Antibody
merck millipore,默克密理博,CBL202,Anti-Vimentin Antibody, clone VIM 3B4