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merck millipore,默克密理博,CBL202,Anti-Vimentin Antibody, clone VIM 3B4
产品名称：Anti-Vimentin Antibody, clone VIM 3B4
产品型号：CBL202
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
merck millipore,默克密理博,CBL202,Anti-Vimentin Antibody, clone VIM 3B4
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Mk, Am, B, Ca, Ch, H WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 CBL202 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Vimentin Antibody, clone VIM 3B4 产品信息 格式 Purified 控制 - Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
演示 Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. 应用 应用 This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Vimentin purified from bovine lens 克隆 VIM 3B4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells. 同种型 IgG2a 品种反应性 MonkeyAmphibianBovineCanineChickenHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003380.2
Entrez基因汇总 Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] 基因符号 - vimentin
- VIM
- FLJ36605
- Vimentin.
纯化方法 Protein A purfied UniProt编号 - P08670
UniProt汇总 FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SIZE: 466 amino acids; 53652 Da
SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2–8°C for up to 12 months from date of receipt. 包装信息 数量 50 µg
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