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merck millipore,默克密理博,MAB3222,Anti-BrdU Antibody, clone IIB5
产品名称：Anti-BrdU Antibody, clone IIB5
产品型号：MAB3222
Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
merck millipore,默克密理博,MAB3222,Anti-BrdU Antibody, clone IIB5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A ICC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3222 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-BrdU Antibody, clone IIB5 Alternate Names - BrdU
背景信息 The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. 产品信息 格式 Purified 控制 - After incorporation of BrdU, all DNA containing species
演示 Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide. 应用 应用 Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P). 主要应用 - Immunocytochemistry
- Flow Cytometry
应用说明 Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)生物信息 免疫原品种 A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin. 克隆 IIB5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA. 同种型 IgG1 品种反应性 All 抗体类型 Monoclonal Antibody 纯化方法 Protein A purfied 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,AG240,Synuclein α, control peptide for AB5334P
下一件merck millipore产品：merck millipore,默克密理博,370679,Guanosine 3ʹ,5ʹ-cyclic Monophosphorothioate, β-Phenyl-1,N²-etheno-8-bromo-, Rp-Isomer, Sodium Salt - CAS 172806-20-1 - Calbiochem
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