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品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC R Purified Monoclonal Antibody 描述 产品目录编号 MAB1982 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin α6 Antibody, clone MA6 Alternate Names - CD49f
产品信息 格式 Purified 演示 Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide. 应用 应用 This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6. 主要应用 - Immunohistochemistry
- Flow Cytometry
不建议的应用 - Western Blotting
应用说明 Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL.
Optimal working dilutions must be determined by end user.
Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.生物信息 克隆 MA6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes. 同种型 IgG2κ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001079818.1
- NM_000210.2
Entrez基因汇总 The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - CD49f
- ITGA6
- VLA-6
- ITGA6B
UniProt编号 - P23229
UniProt汇总 FUNCTION: SwissProt: P23229 # Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
SIZE: 1130 amino acids; 126619 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
PTM: Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
DISEASE: "SwissProt: P23229 # Defects in ITGA6 are a cause of epidermolysis bullosa with pyloric atresia (EB-PA) [MIM:226730]; also known as aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive disease characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus."
SIMILARITY: SwissProt: P23229 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at +2-8°C for up to 12 months. 包装信息 数量 100 µg
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