merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1965,Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

  • merck millipore,默克密理博,MAB1965,Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

    产品名称：Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)
    产品型号：MAB1965
    This Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) is validated for use in FC, IH, IP, FUNC, WB for the detection of Integrin β1.

    merck millipore,默克密理博,MAB1965,Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

  • 产品介绍
  • merck millipore,默克密理博,MAB1965,Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWB, FC, IHCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB1965
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)
    Alternate Names
    • CD29
    背景信息 The primary function of the integrin family is to mediate cell-cell and cell-matrix adhesion interaction. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. Loss or downregulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. Furthermore, loss or downregulation of integrin occurs relatively frequently in poorly differentiated colorectal and breast carcinomas as determined by immunohistochemistry.
    产品信息
    格式 Ascites
    控制
    • Human lung, liver, and skeletal muscle tissues
    演示 Ascites fluid containing no preservatives.
    应用
    应用 This Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) is validated for use in FC, IH, IP, FUNC, WB for the detection of Integrin β1.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunohistochemistry
    应用说明 Inhibition of Jurkat binding to collagen and fibronectin:
    1:100 Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells with JB1A.

    Immunoprecipitation: 1:250-500

    Western blotting (reducing, 115 kDa; non-reducing, 130 kDa): 1:1000-2000

    Note: binding of antibody may be inhibited by magnesium ion concentrations exceeding 0.5mM.

    Immunocytochemistry/Immunohistochemistry: JB1A can be used on unfixed cells in flow cytometry and culture {Akimov, 2001; Blood 98(5):1567-1576}. Often times fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtain strong signals since it fixes the primary antibodies to the tissues.

    FACS: JB1A has been primarily used against live cells at 1:200-1:500 followed by goat anti-mouse conjugated secondaries

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Purified beta 1 integrin and Jurkat cells
    表位 a.a. 82-87
    克隆 JB1A (a.k.a. J10)
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with the human integrin beta 1. Note: integrin beta 1 is expressed in all tissues/cell types except erythrocytes. The epitope recognized by JB1a was recently mapped to residues 82-87 of the mature beta 1 integrin chain. In this study Mn++ was found to inhibit binding of JB1a both to mature beta 1 integrin and to a synthetic peptide corresponding to residues 82-87{Ni, 1998}.
    同种型 IgG1
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_133376.1
    • NM_002211.2
    • NM_033666.1
    • NM_033667.1
    • NM_033668.1
    • NM_033669.1
    Entrez基因汇总 Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
    基因符号
    • MSK12
    • ITGB1
    • GPIIA
    • OTTHUMP00000063731
    • OTTHUMP00000046253
    • OTTHUMP00000063733
    • VLAB
    • OTTHUMP00000063732
    • MDF2
    • FNRB
    • CD29
    纯化方法 Unpurified
    UniProt编号
    • P05556
    UniProt汇总 FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

    SIZE: 798 amino acids; 88465 Da

    SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

    SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

    TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).

    SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB1965,Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

上一件merck millipore产品：merck millipore,默克密理博,04-855,Anti-GluR1 Antibody, clone C3T, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,AB3468,Anti-Heat Shock Protein 90β Antibody


相关产品

merck millipore,默克密理博,SCR002,ES Cell Marker Sample Kit merck millipore,默克密理博,SCR002,ES Cell Marker Sample Kit

merck millipore,默克密理博,06-556,Anti-Kip2 Antibody merck millipore,默克密理博,06-556,Anti-Kip2 Antibody

merck millipore,默克密理博,ABE62,Anti-MYSM1/2A-DUB Antibody merck millipore,默克密理博,ABE62,Anti-MYSM1/2A-DUB Antibody

merck millipore,默克密理博,ABE401,Anti-BCL-11A Antibody merck millipore,默克密理博,ABE401,Anti-BCL-11A Antibody

merck millipore,默克密理博,04-855,Anti-GluR1 Antibody, clone C3T, rabbit monoclonal merck millipore,默克密理博,04-855,Anti-GluR1 Antibody, clone C3T, rabbit monoclonal

merck millipore,默克密理博,AB3468,Anti-Heat Shock Protein 90β Antibody merck millipore,默克密理博,AB3468,Anti-Heat Shock Protein 90β Antibody

merck millipore,默克密理博,476475,PKC Inhibitor, EGF-R Fragment 651-658, Myristoylated - Calbiochem merck millipore,默克密理博,476475,PKC Inhibitor, EGF-R Fragment 651-658, Myristoylated - Calbiochem

merck millipore,默克密理博,ABN346,Anti-LGI4 Antibody merck millipore,默克密理博,ABN346,Anti-LGI4 Antibody

merck millipore,默克密理博,539215,Protein C, Human Plasma merck millipore,默克密理博,539215,Protein C, Human Plasma

merck millipore,默克密理博,07-1282,Anti-phospho-PRAS40 (Thr246) Antibody merck millipore,默克密理博,07-1282,Anti-phospho-PRAS40 (Thr246) Antibody


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部