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品种反应性 主要应用 宿主 格式 抗体类型 B, M, R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1282 描述 Anti-phospho-PRAS40 (Thr246) Antibody Alternate Names - proline-rich Akt substrate, 40 kDa
- 40 kDa proline-rich AKT substrate
- AKT1 substrate 1 (proline-rich)
背景信息 PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1. 产品信息 格式 Affinity Purified 演示 Antigen affinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-phospho-PRAS40 (Thr246) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40. 表位 Phosphorylated Thr246 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects PRAS40 when phosphorylated on Thr246. 品种反应性 BovineMouseRatHuman Species Reactivity Note Rat and Mouse. Predicted to cross-react with human and bovine based sequence homology of immunizing peptide. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_026270.3
基因符号 - PRAS40
- Lobe
- MGC2865
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - Q96B36
UniProt汇总 FUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm › cytosolBy similarity. Note= Found in the cytosolic fraction of the brain By similarity.
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
产品使用声明 质量保证 Evaluated by western blot on untreated and Insulin-treated NIH/3T3 cell lysate
Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect PRAS40 in Insulin-treated NIH/3T3 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. 包装信息 数量 100 µg
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