- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB1592,Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1
产品名称：Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1
产品型号：MAB1592
Detect Neurofilament H & M using this Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 validated for use in ELISA, FC, RIA, WB, IC, IH(P).
merck millipore,默克密理博,MAB1592,Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, Ch, H ICC, ELISA, FC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MAB1592 Replaces 04-1061 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section. 产品信息 格式 Ascites 演示 Liquid. Contains no preservative. 应用 应用 Detect Neurofilament H & M using this Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 validated for use in ELISA, FC, RIA, WB, IC, IH(P). 主要应用 - Immunocytochemistry
- ELISA
- Flow Cytometry
- Western Blotting
应用说明 Western blot : recognizes, 200kDa, and 150kDa NF-H and NF-M bands primarily. Staining is removed with phosphatase treatment. Because NF-L also contains lysine-serine PO4 sites, NP1 may cross react with NF-L as well, but this has not be substantiated in traditional westerns.
Immunohistochemistry. Works on paraffin embedded, formalin fixed and frozen acteone fixed tissue sections. 1:50-1:500 for immunofluorescence.
Immunocytochemistry on PC12, TERA-2 and other neuronal lines.
FACS ELISA/RIA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Bovine neurofilaments 表位 phosphorylated 克隆 NP1 宿主 Mouse 特异性 Recognizes the KSP repeats of the phosphorylated forms of neurofilament-M (NF-M) and neurofilament-H (NF-H), because NF-L does contain a few of these Lysine-serine-PO4-proline repeats some cross reactivity to NF-L may be seen at very high antibody concentrations. 同种型 IgG1 品种反应性 RatBovineChickenHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_021076.2
基因符号 - KIAA0845
- NEFH
- NFH
- NF-H
修改 - Phosphorylation
UniProt编号 - P07197
- P12036
UniProt汇总 FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,05-1538,Anti-MAGOH Antibody, clone 21B12
下一件merck millipore产品：merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
相关产品
merck millipore,默克密理博,SCC036,Human Mesenchymal Stem Cells (derived from hES cells)
merck millipore,默克密理博,07-608,Anti-dimethyl Histone H3 (Lys23) Antibody
merck millipore,默克密理博,MAB2148F,Anti-PECAM-1 Antibody, clone P2B1, FITC conjugated
merck millipore,默克密理博,05-1538,Anti-MAGOH Antibody, clone 21B12
merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
merck millipore,默克密理博,07-2188,Anti-phospho LRP-6 Antibody (Ser1607)
merck millipore,默克密理博,ABD50,Anti-BMPR-1B Antibody
merck millipore,默克密理博,04-1100,Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal
