merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
产品型号：03-103
This RIPAb+ SNRNP70 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-103,RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
B, H, Ca, M, R	WB

描述
产品目录编号	03-103
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction. The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.

产品信息
格式	Purified
控制	Included negative control rabbit IgG antibody and control primers specific for U1snRNA.
演示	Anti-SNRNP70 (Rabbit Polyclonal IgG) One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C. Normal Rabbit IgG One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, U1snRNA One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C. FOR: GGG AGA TAC CAT GAT CAC GAA GGT REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

应用
应用	This RIPAb+ SNRNP70 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).

生物信息
免疫原品种	The SNRNP70 purified antibody is made against a synthetic peptide corresponding to a region of human SNRNP70.
宿主	Rabbit
特异性	SNRNP70
品种反应性	BovineHumanCanineMouseRat
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001009820
基因符号	SNRPN SNRNP70 U1SNRNP RNPU1Z RPU1 U1AP1 U1SNRNP70
纯化方法	Purified
UniProt编号	P08621
UniProt汇总	FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA. SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2. SUBCELLULAR LOCATION: Nucleus. POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked. Extensively phosphorylated on serine residues in the C-terminal region. INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus. SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700). Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700).
Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.