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merck millipore,默克密理博,05-1538,Anti-MAGOH Antibody, clone 21B12
产品名称：Anti-MAGOH Antibody, clone 21B12
产品型号：05-1538
Use Anti-MAGOH Antibody, clone 21B12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect MAGOH also known as mago-nashi (Drosophila) homolog proliferation-associated.
merck millipore,默克密理博,05-1538,Anti-MAGOH Antibody, clone 21B12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1538 描述 Anti-MAGOH Antibody, clone 21B12 Alternate Names - mago-nashi homolog, proliferation-associated (Drosophila)
- mago-nashi (Drosophila) homolog, proliferation-associated
- mago-nashi homolog
背景信息 MAGOH is a component of the splicing-dependent multiprotein exon junction complex (EJC) found at splice junctions on mRNAs. The EJC core complex contains CASC3, EIF4A3, MAGOH and Y-14 and assembles approximately 20 nucleotides upstream of exon-exon junctions. This complex functions in post-splicing events, such as nuclear export and nonsense-mediated decay of mRNA. Core components of the EJC remain bound to spliced mRNAs throughout all stages of mRNA metabolism and mark the position of the exon-exon junctions in mature mRNA. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-MAGOH Antibody, clone 21B12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect MAGOH also known as mago-nashi (Drosophila) homolog proliferation-associated. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation:
Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody.
Immunocytochemistry:
Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human MAGOH. 表位 Unknown 克隆 21B12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes MAGOH. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002361.1
Entrez基因汇总 Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq]. 基因符号 - MAGOHA
- MAGOH
纯化方法 Protein G purfied UniProt编号 - P61326
UniProt汇总 FUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
SUBUNIT STRUCTURE: Heterodimer with RBM8A. Part of the EJC core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, RBM8A, RNPS1, SRRM1, NXF1, SAP18, UPF3B, UPF2 and THOC4. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Detected in granule-like structures in the dendroplasm by similarity. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
TISSUE SPECIFICTY: Ubiquitous.
SEQUENCE SIMILARITIES: Belongs to the mago nashi family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MAGOH on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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