merck millipore,默克密理博,04-1100,Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

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merck millipore,默克密理博,04-1100,Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal
产品名称：Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal
产品型号：04-1100
Anti-Smad1 Antibody, clone EP565Y is a Rabbit Monoclonal Antibody for detection of Smad1 also known as MAD-mothers against decapentaplegic-homolog 1 & has been validated in ICC & WB.

merck millipore,默克密理博,04-1100,Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1100,Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1100
描述	Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal
Alternate Names	transforming growth factor-beta signaling protein 1 MAD, mothers against decapentaplegic homolog 1 MAD, mothers against decapentaplegic homolog 1 (Drosophila) Mad-related protein 1 Mothers against DPP homolog 1 SMAD 1 SMAD family member 1 SMAD, mothers against DPP homolog 1 SMAD, mothers against DPP homolog 1 (Drosophila) Sma- and Mad-related protein 1 TGF-beta signaling protein 1 Transforming growth factor-beta-signaling protein 1
背景信息	Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-Smad1 Antibody, clone EP565Y is a Rabbit Monoclonal Antibody for detection of Smad1 also known as MAD-mothers against decapentaplegic-homolog 1 & has been validated in ICC & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected Smad1 in HeLa cells.

生物信息
免疫原品种	Synthetic peptide from human Smad1.
克隆	EP565Y
宿主	Rabbit
特异性	This antibody recognizes Smad1.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001003688
Entrez基因汇总	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
基因符号	hSMAD1 BSP-1 BSP1 CMT2D GARS HMN5 JV4-1 JV41 MADH1 MADR1
纯化方法	Unpurified
UniProt编号	Q15797
UniProt汇总	FUNCTION: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SUBUNIT STRUCTURE: Interacts with HGS, NANOG and ZCCHC12 By similarity. May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. TISSUE SPECIFICITY:Ubiquitous. Highest expression seen in the heart and skeletal muscle. PTM: Phosphorylated on serine by BMP type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. SEQUENCE SIMILARITIES: Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect Smad1 in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.