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merck millipore,默克密理博,HTS208M,ChemiSCREEN™ Membrane Preparation Recombinant Human AT2 Angiotensin II Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human AT2 Angiotensin II Receptor
产品型号：HTS208M
Human AT2 GPCR membrane preparation for Radioligand binding Assays.
merck millipore,默克密理博,HTS208M,ChemiSCREEN™ Membrane Preparation Recombinant Human AT2 Angiotensin II Receptor
- 产品介绍
- merck millipore,默克密理博,HTS208M,ChemiSCREEN™ Membrane Preparation Recombinant Human AT2 Angiotensin II Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay Human Chem-1 AT2 描述 产品目录编号 HTS208M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human AT2 Angiotensin II Receptor 概述 Full-length human AGTR2 cDNA encoding AT2 背景信息 Angiotensin II (Ang II), an octapeptide produced by cleavage of angiotensinogen by angiotensin-converting enzyme, plays a fundamental role in cardiovascular homeostasis. Two GPCRs, AT1 and AT2, mediate the effects of AngII. Most of the known actions of angiotensin II are mediated through the AT1 receptor and serve to maintain blood pressure and glomerular filtration rate in the face of extracellular volume depletion (Griendling et al. 1996). In vitro and in vivo studies indicated that the AT2 receptor counterbalances the effect of the AT1 receptor (Horiuchi et al. 1999). Expression of the AT2 receptor is developmentally regulated: it is highly expressed in various fetal tissues and at a lower density in adult adrenal medulla, brain, and reproductive tissues (Griendling et al. 1996). AT2 expression appears to be re-expressed or up-regulated after vascular injury, myocardial infarction, cardiac failure or wound healing (Matsubara 1998). Millipore's AT2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of AT2. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-CGP42112. With 0.25 nM [125I]-CGP42112, 5μg/well AT2 Membrane Prep typically yields greater than 50-fold signal-to-background ratio. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C应用 应用 Human AT2 GPCR membrane preparation for Radioligand binding Assays. 应用说明 Radioligand binding assay 生物信息 品种 Human Entrez基因编号 - NM_000686.4
Entrez基因汇总 Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G-protein coupled receptors. It is an intergral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame. 基因符号 - AT2
- AGTR2
- MRX88
- ATGR2
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 AT2 靶子系列 Angiotensin UniProt编号 - P50052
UniProt汇总 FUNCTION: SwissProt: P50052 # Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
SIZE: 363 amino acids; 41184 Da
SUBUNIT: Interacts with MTUS1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
SIMILARITY: SwissProt: P50052 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-CGP42112. (Perkin Elmer # NEX-324 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 50-fold signal:background with 125I labeled CGP42112 at 0.25 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with AT2 Receptor membrane prep.
5 µg/well Signal:Background 148.1 Specific Binding (cpm) 17301
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax for [125I]-CGP42112 binding: 3.9 pmol/mg protein
Kd for [125I]-CGP42112 binding: ~0.32 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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