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merck millipore,默克密理博,HTS176RTA,Ready-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells
产品名称：Ready-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells
产品型号：HTS176RTA
Human S1P1 / EDG1 GPCR Frozen Cells for calcium flux FLIPR Assays.
merck millipore,默克密理博,HTS176RTA,Ready-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells
- 产品介绍
重要规格表
品种 宿主细胞 蛋白靶 Human Chem-4 S1P1 / EDG1 描述 产品目录编号 HTS176RTA 商名 - Ready-to-Assay
描述 Ready-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells 背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that transmits signals through a family of five G-protein-coupled receptors to regulate cell proliferation, migration, cytoskeletal organization, and differentiation. S1P1 was the first identified S1P receptor. It primarily couples to PTX-sensitive Gi/o proteins and mediates S1P-induced adenylate cyclase inhibition. Expression of S1P1 is pervasive, including spleen, brain, heart, lung, adipose tissues, liver, thymus, kidney, and skeletal muscle (Zhang et al. 1999). The deletion of S1P1 in mice results in embryonic lethality (Liu et al., 2000) with death attributable to incomplete vascular maturation. Recent reports demonstrate specific roles for S1P1 in lymphocyte recirculation/egress (Matloubian et al., 2004). Chemicon's cloned human S1P1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P1 and its ligands.产品信息 部件 - Fifty (50) mL of Media Component.
- Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
应用 应用 Human S1P1 / EDG1 GPCR Frozen Cells for calcium flux FLIPR Assays. 生物信息 品种 Human GPCR类别 A 宿主细胞 Chem-4 蛋白靶 S1P1 / EDG1 靶子系列 Lysophospholipid 细胞系类型 - Ready-to-Assay
产品使用声明 销售限制 This product requires a User Agreement (Label License). Please see product datasheet for further information. 使用声明 - This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Place cells in liquid nitrogen immediately upon receipt. 包装信息 数量 1 kit
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