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merck millipore,默克密理博,HTS090M,ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
产品名称：ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
产品型号：HTS090M
Human OT GPCR membrane prepation for Radioligand binding Assays.
merck millipore,默克密理博,HTS090M,ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
- 产品介绍
- merck millipore,默克密理博,HTS090M,ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
重要规格表
品种 宿主细胞 蛋白靶 Human Chem-1 OT 描述 产品目录编号 HTS090M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation 概述 Human Oxytocin Receptor 背景信息 Oxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Chemicon's oxytocin membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of oxytocin receptor interactions with oxytocin. The membrane preparations exhibit a Kd of 0.53nM for [125I]-OVTA. With 5.0 ug/well Oxytocin Membrane Prep and 0.5 nM [125I]-OVTA, a greater than 6-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 6-fold signal:background with 125I-labeled OVTA at 0.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human OT GPCR membrane prepation for Radioligand binding Assays. 生物信息 品种 Human Entrez基因编号 - NM_000916.3
Entrez基因汇总 The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition. 基因符号 - OT-R
- OXTR
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 OT 靶子系列 Oxytocin UniProt编号 - P30559
UniProt汇总 FUNCTION: SwissProt: P30559 # Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system.
SIZE: 389 amino acids; 42772 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P30559 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] OVTA (Perkin Elmer # NEX254)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Oxytocin Receptor membrane prep:
5 µg/well 10 µg/well Signal:Background 9 9 Specific Binding (cpm) 21871 24926
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 4.5 pmol/mg
Kd: 0.53 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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