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merck millipore,默克密理博,MAB3747-I,Anti-Microphthalmia (Mi) Antibody, clone C5
产品名称：Anti-Microphthalmia (Mi) Antibody, clone C5
产品型号：MAB3747-I
Use Anti-Microphthalmia (Mi) Antibody, clone C5 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor.
merck millipore,默克密理博,MAB3747-I,Anti-Microphthalmia (Mi) Antibody, clone C5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3747-I 描述 Anti-Microphthalmia (Mi) Antibody, clone C5 Alternate Names - bHLHe32
- Microphthalmia-associated transcription factor
- Class E basic helix-loop-helix protein 32
背景信息 MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts. 产品信息 格式 Purified 控制 - Mouse brain tissue lysates
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-Microphthalmia (Mi) Antibody, clone C5 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Imunohistochemistry Analysis: A representative lot was used by an independent laboratory in human adrenal gland tumor tissue. (Lau, S. (2012). Pathology – Research and Practice 208:113– 117.) 生物信息 免疫原品种 Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. 表位 N-terminus 克隆 C5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 In Western blotting, it recognizes a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. 同种型 IgG1κIgG2aκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Mouse. Predicted to react with Human based on immunogen design. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000239
基因符号 - MiTF
- MITF
- BHLHE32
纯化方法 Protein G purified UniProt编号 - O75030
产品使用声明 质量保证 Evaluated by Western Blot on mouse brain tissue lysates.
Western Blot Analysis: A 1:500 dilution of this antibody detected Microphthalmia on 10 ug of mouse brain tissue lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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