merck millipore,默克密理博,04-236,Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal

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merck millipore,默克密理博,04-236,Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal
产品名称：Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal
产品型号：04-236
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Nibrin/NBS1 Antibody, clone Y112 detects level More>>

merck millipore,默克密理博,04-236,Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-236,Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, ICC, IHC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-236
品牌系列	Upstate
商名	Upstate
描述	Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal
Alternate Names	p95 protein of the MRE11/RAD50 complex Cell cycle regulatory protein p95 Nijmegen breakage syndrome 1 (nibrin) Nijmegen breakage syndrome protein 1
背景信息	Nibrin (NBS1, NBN) is known to be a key regulator of DNA Damage repair by forming a complex with Rad50 and MRE11. This complex, more commonly referred to as MRN (Mre11/Rad50/Nibrin) is important in double-stranded break repair, DNA recombination, cell cycle checkpoint control, and meiosis. This complex quickly recognizes double stranded break (DSB) sites and quickly relocates to these sites. Nibrin senses DNA damage by recruiting ATM, ATR, and DNA-PK to the damage sites and activation their functions.

产品信息
格式	Culture Supernatant
控制	HeLa whole cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Nibrin/NBS1 Antibody, clone Y112 detects level of Nibrin/NBS1 & has been published & validated for use in IC, IH, IH(P), IP & WB.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry: A 1:50 dilution of a previous lot was used in IH. Immunoprecipitation: A 1:60 dilution of a previous lot was used in IP. Immunocytochemistry: A 1:50 dilution of a previous lot was used in IC.

生物信息
免疫原品种	KLH conjugated to Nibrin/NBS1 linear peptide.
表位	C-terminus
克隆	Y112
宿主	Rabbit
特异性	This antibody recognizes Nibrin/NBS1.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Proven to react with human and mouse. Not expected to react with rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002485.4
Entrez基因汇总	Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]
基因符号	nibrin AT-V1 AT-V2 ATV FLJ10155 MGC87362 NBS NBS1 P95
纯化方法	Unpurified
UniProt编号	O60934
UniProt汇总	Function: Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Ref.9 Ref.14 Ref.19 Subunit structure: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A By similarity. Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with HJURP, KPNA2 and TERF2. Subcellular location: NucleusBy similarity. TelomereBy similarity. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity. Tissue specificity: Ubiquitous. Expressed at high levels in testis. Domain: The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. Ref.18 The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. Ref.18 The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. Post-translational modification: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. Involvement in disease: Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies.Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). Miscellaneous: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. Sequence similarities: Contains 1 BRCT domain. Contains 1 FHA domain. Sequence caution: The sequence AAI08651.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 550.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HeLa whole cell lysate. Western Blot Analysis: A 1:250 dilution of this lot was used to detect Nibrin/NBS1 in HeLa whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.