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merck millipore,默克密理博,HTS062M,ChemiScreen™ Recombinant Human NMU1 Neuromedin Receptor
产品名称：ChemiScreen™ Recombinant Human NMU1 Neuromedin Receptor
产品型号：HTS062M
Human NMU1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS062M,ChemiScreen™ Recombinant Human NMU1 Neuromedin Receptor
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 NMU1 描述 产品目录编号 HTS062M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Recombinant Human NMU1 Neuromedin Receptor 概述 Human NMU1 背景信息 Neuromedin U (NmU) is a peptide characterized by its ability to promote smooth muscle contraction (Brighton et al., 2004a). Two GPCRs, NMU1 and NMU2, mediate the contractile effects of neuromedin U by activation of both Gq and Gi (Brighton et al., 2004b). In addition, NmU binds to NMU1 expressed on Th2 cells to induce cytokine release (Johnson et al., 2004). Chemicon's NMU1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NMU1 interactions with neuromedin U25. The membrane preparations exhibit a Kd of 0.08 nM for [125I]-neuromedin U25. With 5 µg/well NMU1 Membrane Prep and 0.5 nM [125I]-neuromedin U25, a greater than 30-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 30-fold signal:background with 125I-labeled neuromedin U25 at 0.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human NMU1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_006056.3
基因符号 - NMU-R1
- NMUR1
- FM3
- (FM-3)
- FM-3
- GPR66
- GPC-R
- NMU1R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 NMU1 靶子系列 Neuromedin U UniProt编号 - Q9HB89
UniProt汇总 FUNCTION: SwissProt: Q9HB89 # Receptor for the neuromedin-U and neuromedin-S neuropeptides (By similarity).
SIZE: 426 amino acids; 47351 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in greatest abundance in peripheral organs, particularly in elements of the gastrointestinal and urogenital systems with highest levels in testes. In central nervous system structures express levels are much lower than those seen in peripheral organs. Within the CNS, has been detected in highest abundance in the cerebellum, dorsal root ganglia, hippocampus, and spinal cord.
SIMILARITY: SwissProt: Q9HB89 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-neuromedin U25 (Perkin Elmer # NEX383)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with NMU1 membrane prep.
10 µg/well 5 µg/well Signal:Background 15.7 20.5 Specific Binding (cpm) 4779 4829
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 1.06 pmol/mg
Kd: 0.08 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 2 units
上一件merck millipore产品：merck millipore,默克密理博,MCYTOMAG-70K-PMX,MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay
下一件merck millipore产品：merck millipore,默克密理博,MAB5778,Anti-NMDAR2B Antibody, CT
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