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描述 产品目录编号 14-745M 品牌系列 Upstate
商名 - Upstate
描述 TAO3 Protein, active, 250 µg 概述 N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411; N47S. 产品信息 演示 10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. 应用 应用 Active, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 纯度 Purity 84% by SDS-PAGE and Coomassie blue staining. 来源 Recombinant, human TAO3 amino acids 1-411; N47S. Expressed by baculovirus in Sf21 cells. 品种 Human 特异活性 915U/mg, where one unit of TAO3 activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30°C with a final ATP concentration of 100μM. Entrez基因编号 - NM_016281.3
基因符号 - FLJ31808
- TAOK3
- JIK
- hKFC-A
- MAP3K18
- DPK
- KDS
- DKFZp666H245
蛋白靶 TAO3 纯化方法 Ni2+/NTA-agarose 靶子系列 STE UniProt编号 - Q9H2K8
UniProt汇总 FUNCTION: SwissProt: Q9H2K8 # Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
SIZE: 898 amino acids; 105406 Da
SUBUNIT: Self-associates.
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also localized to the peripheral cell membrane.
TISSUE SPECIFICITY: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q9H2K8 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of TAO3.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.包装信息 数量 250 µg 包装 Also available in 10 μg size (cat#14-745) and in bulk (cat# 14-745-K).
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