merck millipore,默克密理博,48-615MAG,MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit

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merck millipore,默克密理博,48-615MAG,MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

可用的分析物	品种反应性	主要应用	检测方法
HSP90α, HSP27 (Total), HSP27 (pS78/pS82), HSP60, HSP70	H	Mplex	Luminex xMAP

描述
产品目录编号	48-615MAG
商名	MILLIPLEX
描述	MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay
概述	Heat shock proteins (HSPs) are a family of proteins that function as molecular chaperones within the cell, and have many important roles including folding nascent proteins and maintaining proteins in a folded, active state. They can be induced in response to a variety of stresses including heat shock, radiation, and cytotoxic drug exposure, and have increased expression in cancer cells relative to normal cells. A number of these proteins are being investigated as potential targets for cancer therapy, as they can have effects on proliferation, apoptosis, metastasis, and chemotherapy resistance in certain cancers. The levels of some of the family members are coordinately regulated by the transcription factor Heat Shock Factor 1 (HSF1), in response to a variety of cellular stresses. Interestingly, HSP90 inhibitors can disrupt the interaction of HSP90 with HSF1, which can lead to increased expression of HSP family members such as HSP70 and influence the efficacy of treatment. To better understand the roles and regulation of heat shock proteins and the heat shock response in cancer and other physiological processes, there is increased interest in simultaneous analysis of key heat shock proteins in a panel or multiplex format, rather than studying each protein in isolation.

应用
应用	The analytes available for this multiplex kit are: HSP27 (Total), HSP27 (pS78/pS82), HSP60, HSP70, HSP90α.
主要应用	Multiplexing
应用说明	• An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 1 (provided). • The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).

生物信息
特异性	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
品种反应性	Human
可用的分析物	HSP90α HSP27 (Total) HSP27 (pS78/pS82) HSP60 HSP70

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Recommended storage for kit components is 2 - 8°C.

包装信息
数量	96-well plate
包装	96-well plate

merck millipore,默克密理博,48-615MAG,MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

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