merck millipore,默克密理博,07-888,Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

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merck millipore,默克密理博,07-888,Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
产品名称：Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
产品型号：07-888
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.

merck millipore,默克密理博,07-888,Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

产品介绍

merck millipore,默克密理博,07-888,Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-888
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
Alternate Names	proline-rich Akt substrate, 40 kDa 40 kDa proline-rich AKT substrate AKT1 substrate 1 (proline-rich) proline-rich Akt substrate
背景信息	PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

产品信息
格式	Purified
控制	PDGF stimulated NIH3T3 cells.
演示	Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.

应用
应用	Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.
主要应用	Western Blotting
应用说明	Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.

生物信息
免疫原品种	Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001092102
Entrez基因汇总	AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
基因符号	PRAS40 Lobe MGC2865 OTTHUMP00000196757 OTTHUMP00000196758 OTTHUMP00000196759 OTTHUMP00000196760
修改	Phosphorylation
纯化方法	Affinity Purfied
UniProt编号	Q96B36
UniProt汇总	FUNCTION: SwissProt: Q96B36 # May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection. SIZE: 256 amino acids; 27383 Da SUBUNIT: The phosphorylated form interacts with 14-3-3. SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity). TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).

产品使用声明
质量保证	Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates. Western Blot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.