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merck millipore,默克密理博,HTS056M,ChemiScreen™ Membrane Preparation Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)
产品名称：ChemiScreen™ Membrane Preparation Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)
产品型号：HTS056M
Human FPRL1 / ALX / HM63 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS056M,ChemiScreen™ Membrane Preparation Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)
- 产品介绍
- merck millipore,默克密理博,HTS056M,ChemiScreen™ Membrane Preparation Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 FPRL1 / ALX / HM63 描述 产品目录编号 HTS056M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Membrane Preparation
Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)概述 Human FPRL1 背景信息 FPRL1 (formyl peptide receptor-like 1, also known as FPR2) is a GPCR that belongs to the N-formyl peptide receptor family. Initially described as a receptor for lipoxin A4, FPRL1 has been shown to bind to a synthetic peptide WKYMVm, amyloid beta peptides, and N-formylated mitochondrial peptides and mediates phagocyte chemotaxis (Fiore et al., 1994; Le et al., 1999; Rabiet et al., 2005; Iribarren et al., 2005). Chemicon's FPRL1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of FPRL1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for WKYMVm in a GTPγS binding assay. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to 0.5 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human FPRL1 / ALX / HM63 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 浓缩 0.5 mg/mL 品种 Human 品种反应性 Human Entrez基因编号 - NM_001462.3
- NM_001005738.1
基因符号 - FMLP-R-II
- FPRL1
- RFP
- FPR2A
- FMLPX
- FPR2
- HM63
- FPRH2
- FMLP-R-I
- LXA4R
- FPRH1
- ALXR
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 FPRL1 / ALX / HM63 靶子系列 N-formylpeptide UniProt编号 - P25090
UniProt汇总 FUNCTION: SwissProt: P25090 # Low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
SIZE: 351 amino acids; 38964 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
SIMILARITY: SwissProt: P25090 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled WKYMVm is added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) pre-wetted with water, and washed 2 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4. The plate is dried and counted.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific WKYMVm stimulated [35S]-GTPγS binding when final concentration of 0.3nM [35S]-GTPγS was used.
产品使用声明 质量保证 Kd for WKYMVm: 0.6 nM in a GTPγS binding assay 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 1 mL 包装 200 units
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