merck millipore,默克密理博,HTS022M,ChemiSCREEN™ MC3 Membrane Preparation

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merck millipore,默克密理博,HTS022M,ChemiSCREEN™ MC3 Membrane Preparation
产品名称：ChemiSCREEN™ MC3 Membrane Preparation
产品型号：HTS022M
Human MC3 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

merck millipore,默克密理博,HTS022M,ChemiSCREEN™ MC3 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS022M,ChemiSCREEN™ MC3 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPgammaS binding.	Human	Chem-1	MC3

描述
产品目录编号	HTS022M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ MC3 Membrane Preparation
概述	Human MC3
背景信息	The melanocortins, the peptides alpha-, beta- and gamma-melanocyte-stimulating hormone (MSH), bind to a family of five Gs-coupled GPCR receptors (MCRs) and play important roles in energy balance, reproductive function, pigmentation and inflammation (Gantz and Fong, 2003). MC3R is expressed in the CNS, including the hypothalamus, and appears to play a role in energy homeostasis as indicated by the observation that MC3R-null mice have increased subcutaneous fat, a loss of lean body mass, and reduced energy expenditure (Butler et al., 2000; Chen et al., 2000). Macrophages also express MC3R, which together with MC1R mediates the anti-inflammatory activity of melanocortins in animal models of peritonitis and myocardial infarct (Getting et al., 2003; 2004). Chemicon's MC3R membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of MC3R interactions with melanocortins. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-MSH. With 10.0 mg/well MC3R Membrane Prep and 0.25 nM [125I]-MSH, a greater than 7-fold signal-to-background ratio is obtained.

产品信息
格式	Membranes
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human MC3 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
应用说明	Radioligand binding assay, and GTPgammaS binding.

生物信息
浓缩	0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_019888.2
Entrez基因汇总	This gene encodes a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis. Mutations in this gene are associated with a susceptibility to obesity in humans.
基因符号	MC3-R MC3R MC3
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	MC3
靶子系列	Melanocortin
UniProt编号	P41968
UniProt汇总	FUNCTION: SwissProt: P41968 # Receptor for MSH (alpha, beta and gamma) and ACTH. This receptor is mediated by G proteins which activate adenylate cyclase. SIZE: 360 amino acids; 40073 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain, placental, and gut tissues. SIMILARITY: SwissProt: P41968 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 9 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.Binding buffer: 25 mM Hepes, pH 7.0, 1 mM MgCl2, 1.5 mM CaCl2 2H2O, 100mM NaCl, 0.2% BSA, Protease Inhibitor, filter and stored at 4 °C. Radioligand: [125I] MSH (Perkin Elmer# NEX352) Wash Buffer: 25 mM Hepes, pH 7.0, 1 mM MgCl2, 1.5 mM CaCl2 2H2O, 100mM NaCl, 0.2% BSA, filtered and stored at 4°C. One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled MSH at 0.25 nM

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with MC₃ membrane prep and unlabeled NDP-MSH.

	10 µg/well
Signal:Background	7.7
Specific Binding (cpm)	5073

SPECIFICATIONS:
1 unit = 10 μg
Bmax: 0.77 pmol/mg
Kd: 1.0 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.