merck millipore,默克密理博,HTS020M,ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,HTS020M,ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor
产品型号：HTS020M
Human CB2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

merck millipore,默克密理博,HTS020M,ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

产品介绍

merck millipore,默克密理博,HTS020M,ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay and GTPγS binding	Human	CHO (Chinese hamster ovary cells)	CB2

描述
产品目录编号	HTS020M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ Membrane Preparation Recombinant Human CB₂ Cannabinoid Receptor
概述	Full-length Human CB2
背景信息	Cannabinoid compounds include exogenous drugs such as D9-THC, the main psychoactive component of the plant Cannabis sativa, and endogenous mediators, such as anandamide, that belong to eicosanoid family.The biological effects of cannabinoids are mediated by a family of two Gi-coupled 7-transmembrane receptors, CB1 and CB2. The CB1 receptor is found primarily in brain and mediates the psychoactive effects of cannabinoid ligands. The CB2 receptor is expressed mainly in immune cells, including mast cells and CD40-activated B cells, where it mediates proliferation and inhibition of migration (Howlett et al., 2002). Activation of CB2 inhibits the development of liver fibrosis (Julien et al., 2005). In bone, CB2 is expressed in both osteoblasts and osteoclasts, and functions to prevent bone loss (Ofek et al., 2006). In addition, activation of CB2 has an antinociceptive effect in animal models of neuropathic, inflammatory, and acute pain; this effect is mediated by release of endogenous opioids in the periphery (Ibrahim et al., 2005). Millipore's CB2 membrane preparations are crude membrane preparations made from stable recombinant cell lines with a high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of CB2. The membrane preparations exhibit a Kd of 2.7 nM for [3H]-CP55,940. With 5 μg/well CB2 Membrane Prep and 2.5 nM [3H]-CP55,940, greater than 10-fold signal-to-background ratio is obtained.

产品信息
格式	Membranes
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes proteins were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human CB2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay and GTPγS binding

生物信息
品种	Human
品种反应性	Human
Species Reactivity Note	Human CB2
Entrez基因编号	NM_001841.1
Entrez基因汇总	The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.
基因符号	CX5 CNR2 CB-2 OTTHUMP00000044841 CB2
GPCR类别	A
宿主细胞	CHO (Chinese hamster ovary cells)
蛋白靶	CB2
靶子系列	Cannabinoid
UniProt编号	P34972
UniProt汇总	FUNCTION: SwissProt: P34972 # Involved in cannabinoid-induced CNS effects through G- protein mediated inhibition of adenylate cyclase. Could be a receptor for anandamide. SIZE: 360 amino acids; 39681 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Not found in brain. Found in spleen. SIMILARITY: SwissProt: P34972 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl₂, 1 mM CaCl₂, 0.2% BSA, filtered and stored at 4°C. Radioligand: [³H]-CP55940 (Perkin Elmer #NET1051) Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 3H-labeled CP55,940 at 2.5 nM

产品使用声明

质量保证

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of CB2 membrane prep.

	10 µg/well	5 µg/well
Signal:Background	33.5	25.2
Specific Binding (cpm)	7336	6207

SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 50 pmol/mg
Kd: 2.7 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.

包装信息
数量	200 units

merck millipore,默克密理博,HTS020M,ChemiSCREEN™ Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

上一件merck millipore产品：merck millipore,默克密理博,05-522,Anti-SRC-1 Antibody, clone 1135
下一件merck millipore产品：merck millipore,默克密理博,ABC155,Anti-MDC1 Antibody

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech