- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ox, Ca, R, Sh, Fe IH(P) Rb Serum Polyclonal Antibody 描述 产品目录编号 AB932 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glucagon Antibody 背景信息 ANTIGEN:
Synthetic glucagon bound to keyhold limpet hemocyanin (KLH) with carbodiimide.产品信息 格式 Serum 演示 Liquid rabbit antiserum containing 0.1% sodium azide. 应用 应用 This Anti-Glucagon Antibody is validated for use in IH(P) for the detection of Glucagon. 主要应用 - Immunohistochemistry (Paraffin)
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (6 microns thick) were prepared from a number of species including rat, cat, dog, ox and sheep. Cryostat sections of the above tissues can also be used in the staining procedures.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:500-1:1000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37°C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diaminbenzidine HCI (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.
3. After washing off the excess antiserum, with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of glucagon cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum is this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37°C or 4°C.生物信息 宿主 Rabbit 特异性 The antiserum supplied has been evaluated for its ability to stain cells in the pancreas containing glucagon-like immunoreactivity using both the immunoperoxidase and immunofluorescence procedures. 品种反应性 OxCanineRatSheepFeline 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002054.2
Entrez基因汇总 The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. 基因符号 - glucagon
- GCG
- GRPP
- GLP1
- GLP2
UniProt编号 - P01275
UniProt汇总 FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
上一件merck millipore产品：merck millipore,默克密理博,07-807,Anti-phospho-Met (Tyr1003) Antibody
下一件merck millipore产品：merck millipore,默克密理博,ABT29,Anti-Tensin-3 Antibody
相关产品
merck millipore,默克密理博,227013,CCR4 Antagonist - CAS 864289-85-0 - Calbiochem
merck millipore,默克密理博,441201,MACERASE™ Pectinase, Rhizopus sp.
merck millipore,默克密理博,MAB343,Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4
merck millipore,默克密理博,ABC349,Anti-ABCA7 Antibody
merck millipore,默克密理博,07-807,Anti-phospho-Met (Tyr1003) Antibody
merck millipore,默克密理博,ABT29,Anti-Tensin-3 Antibody
merck millipore,默克密理博,05-522,Anti-SRC-1 Antibody, clone 1135
