merck millipore,默克密理博,HTS017M,ChemiSCREEN™ C5aR Membrane Preparation, recombinant

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay (See images & graphs link)	Human	Chem-1	C5aR

描述
产品目录编号	HTS017M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ C5aR Membrane Preparation, recombinant
概述	Full-length human C5aR cDNA
背景信息	C5a is a proinflammatory peptide generated through activation of the complement system, and is more potent than the other anaphylatoxins, C4a and C3a, in activating peripheral blood leukocytes (Gerard & Gerard, 1994). It can actively participate in the regulation of local cytokine network by stimulating production of proinflammatory cytokines (Buchner et al., 1995; Hsu, et al., 1999). The proinflammatory effects of C5a are mediated through binding to a specific 7-TM chemoattractant receptor, C5aR (CD88). Binding of C5a to phagocyte C5aR induces chemotaxis, production of superoxide anions, and release of degradative enzymes. Pharmacologic or genetic disruption of C5a/C5aR interaction reduces sepsis (Riedemann et al., 2003), immune complex-induced lung disease (Shushakova et al., 2002), and Arthrogen-induced arthritis (Grant et al., 2002) in experimental models. Chemicon's cloned human C5a receptor membrane preparations are ideal tools for screening for antagonists of C5a/C5aR interactions. The C5aR Membrane Preps bind specifically to 125I-labeled ligand C5a, with Kd of 0.59 nM. With 5 μg membrane per well and 0.1 nM 125I-labeled C5a, more than 15-fold signal:background was obtained.

产品信息
格式	Membranes
演示	Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 1000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of I-125 labeled C5a (0.1 nM input concentration).

应用
应用	Human C5aR GPCR membrane preparation for Radioligand binding Assays.
应用说明	Radioligand binding assay (See images & graphs link)

生物信息
浓缩	0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_001736.3
基因符号	C5a-R C5AR1 CD88 C5AR C5A C5R1 C5aR
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	C5aR
靶子系列	Anaphylotoxin
UniProt编号	P21730
UniProt汇总	FUNCTION: SwissProt: P21730 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. SIZE: 350 amino acids; 39321 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. SIMILARITY: SwissProt: P21730 ## Belongs to the G-protein coupled receptor 1 family.

产品使用声明
质量保证	SPECIFICATIONS: Bmax for [¹²⁵I]C5a binding: 9.7 pmol/mg protein Kd for [125I] C5a binding: ~ 0.59 nM
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

包装信息
数量	200 assays

merck millipore,默克密理博,HTS017M,ChemiSCREEN™ C5aR Membrane Preparation, recombinant

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