- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M, Po WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB1952P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin β1 Antibody, cytosolic Alternate Names - CD29
产品信息 格式 Purified 控制 - U251 cell line Skin (Basement Membrane)
演示 Protein A Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide. 应用 应用 Detect Integrin β1 using this Anti-Integrin β1 Antibody, cytosolic validated for use in IP & WB. 主要应用 - Western Blotting
应用说明 Western blot: 1-2μg/mL for detection in whole cell lysate. The antibody is effective against reduced (MW = 130kDa) and non-reduced (MW = 110kDa) proteins.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide corresponding to the C-terminus of human integrin beta 1 (a.a. CTTVVNPKYEGK) 表位 cytosolic 宿主 Rabbit 特异性 Reactive with human, mouse, and rat integrin beta-1, as confirmed by western blot. Not reactive with other integrins. 品种反应性 RatHumanMousePig 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_133376.1
- NM_002211.2
- NM_033666.1
- NM_033667.1
- NM_033668.1
- NM_033669.1
Entrez基因汇总 Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. 基因符号 - MSK12
- ITGB1
- GPIIA
- VLAB
- OTTHUMP00000063732
- MDF2
- FNRB
- OTTHUMP00000063731
- OTTHUMP00000046253
- CD29
- OTTHUMP00000063733
纯化方法 Protein A purfied UniProt编号 - P05556
UniProt汇总 FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 798 amino acids; 88465 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.
TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,FCMAB204F,Milli-Mark™ Anti-CD66abce-FITC Antibody, clone Kat4c
下一件merck millipore产品：merck millipore,默克密理博,06-549,Anti-Myc Tag Antibody
相关产品
merck millipore,默克密理博,375115,Heparin Cofactor II, Human Plasma
merck millipore,默克密理博,SLHV013SL,Millex-HV 过滤器，0.45 µm，PVDF，13 mm，环氧乙烷灭菌
merck millipore,默克密理博,567805,Src Kinase Inhibitor I - CAS 179248-59-0 - Calbiochem
merck millipore,默克密理博,SF-496,miR-18a-5p; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,FCMAB204F,Milli-Mark™ Anti-CD66abce-FITC Antibody, clone Kat4c
merck millipore,默克密理博,06-549,Anti-Myc Tag Antibody
merck millipore,默克密理博,14-383,Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg
merck millipore,默克密理博,14-652M,SIK Protein, active, 250 µg
merck millipore,默克密理博,09-722,Anti-XBP1 Antibody
merck millipore,默克密理博,HTS017M,ChemiSCREEN™ C5aR Membrane Preparation, recombinant