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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-722 描述 Anti-XBP1 Antibody Alternate Names - X-box-binding protein-1
- Tax-responsive element-binding protein 5
- X-box binding protein 1
背景信息 X-box Protein 1 (XBP1) is a transcription factor that belongs to the bZIP family. XBP1 plays an important role in the functioning of the immune system and in the cellular stress response. It is essential for the differentiation of plasma cells and is involved in the regulation of the MHC class II genes. XBP1 becomes initiated during an endoplasmic reticulum (ER) stress response by binding to the unfolded protein response element (UPRE) which activates the UPR target genes. Mutations in XBP1 have been associated with mental disorders. 产品信息 格式 Affinity Purified 控制 - LNCAP cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-XBP1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect XBP1 also known as X-box binding protein 1. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 6 µg/ml from a representative lot detected XBP1 on 10 µg of LNCAP cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human XBP1 at and around the N-terminus. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes XBP1 at the C-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Peptide homology with rat and mouse is 86%. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005071
Entrez基因汇总 This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. 基因符号 - XBP2
- XBP1
- TREB5
- XBP-1
纯化方法 Affinity Purfied UniProt编号 - P17861
UniProt汇总 FUNCTION: Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress.
INVOLVEMENT IN DISEASE: Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
SEQUENCE SIMILARITIES: Belongs to the bZIP family.
Contains 1 bZIP domain产品使用声明 质量保证 Evaluated by Western Blot in LNCAP cell lysate.
Western Blot Analysis: 2 µg/ml of this antibody detected XBP1 on 10 µg of LNCAP cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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