merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit

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merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit
产品名称：EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit
产品型号：17-10523
EZ-Magna Nuclear RIP (Native) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.

merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit

产品介绍

merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit

描述
产品目录编号	17-10523
商名	Magna Nuclear RIP
描述	EZ-Magna Nuclear RIP™ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit
概述	Features and Advantages Generates native chromatin to allow analysis of a variety of chromatin-associated RNAs Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 100,000 cells (native) Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios Suitable for analysis by RT-qPCR or RIP-seq Complete set of reagents and detailed protocol to enable first-time success Includes positive and negative control antibodies and qPCR primers Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq). Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. In contrast, nuclear RIP with cross-linked chromatin can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. For less well understood proteins and protein complexes often both approaches are used. The kit described here is for the native approach. If a cross-linked approach is of interest please visit the product page for the Magna Nuclear RIP (Cross-Linked) Kit , catalogue # 17-10520 or the EZ-Magna Nuclear RIP (Cross-Linked) Kit, catalogue # 17-10521.

产品信息
部件	U1 snRNA Negative Control Primers Nuclei Isolation Buffer RIP Lysis Buffer Protein A/G Magnetic Beads 0.5M EDTA DNase I (RNase Free) 2 U/μL DNase I Reaction Buffer Protease Inhibitor Cocktail III, Animal Free RNAse Inhibitor Control Antibodies and Primers Normal Mouse IgG Negative Control Antibody Anti-EZH2 Positive Control Antibody NEAT1 Positive Control Primers
演示	Two boxes containing key reagents for generation of native nuclear lysates and performance of 24 individual RNA-binding protein immunoprecipitation (RIP) reactions. Plus box containing positive and negative control antibodies and qPCR primers.

应用
应用	EZ-Magna Nuclear RIP (Native) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
主要应用	Protein Interaction Assays RNA Binding Protein Immunoprecipitation (RIP)

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.

包装信息
数量	24 assays
包装	Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a native (non-cross-linked) nuclear lysate

merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit

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merck millipore,默克密理博,17-10523,EZ-Magna Nuclear RIP™ (Native)Nuclear RNA-Binding Protein Immunoprecipitation Kit

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