merck millipore,默克密理博,539720,ProteoExtract Tissue Dissociation Buffer Kit

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merck millipore,默克密理博,539720,ProteoExtract Tissue Dissociation Buffer Kit
产品名称：ProteoExtract Tissue Dissociation Buffer Kit
产品型号：539720

merck millipore,默克密理博,539720,ProteoExtract Tissue Dissociation Buffer Kit

产品介绍

merck millipore,默克密理博,539720,ProteoExtract Tissue Dissociation Buffer Kit

描述
概述	The ProteoExtract® Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue. The isolated cells can then be used for total, subcellular, membrane, or other form of proteome extractions. This kit serves as an accessory product for the following kits: ProteoExtract® Subcellular Proteome Extraction Kit (Cat. No.: 539790), ProteoExtract® Subcellular Proteome Extraction Kit, Mini (Cat. No.: 539791), ProteoExtract® Native Membrane Protein Extraction Kit (Cat. No.: 444810).
产品目录编号	539720
品牌系列	Calbiochem®
应用数据	Tissue dissociation of rat pancreatic tissue and human pancreatic tissue was carried out according to the protocol outlined above. 1.3 x 10⁷ dissociated cells were extracted using the ProteoExtract^® Subcellular Proteome Extraction Kit protocol (Cat. No. 539790), incorporating the protocol changes as outlined above. Proteins from the cytosolic fraction, the membrane fraction, the nuclear fraction, and the cytoskeletal fraction were separated by SDS-PAGE, transferred to PVDF membrane, and probed with antibodies to the indicated markers.

产品信息
形式	10 Dissociation reactions, 1 g each
试剂盒包含	10X Tissue Dissociation Buffer, Protease Inhibitor Cocktail, Tissue Dissociation Buffer Additive and a user protocol.

生物信息
样品类型	Fresh tissue samples from eukaryotic organisms

产品使用声明
预定用途	The Calbiochem^® ProteoExtract^® Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue for the purpose of protein extraction. It is an accessory product for the following kits: • ProteoExtract^® Subcellular Proteome Extraction Kit (S-PEK, Cat. No. 539790) • ProteoExtract^® Subcellular Proteome Extraction Kit, Mini (S-PEK Mini, Cat. No. 539791) • ProteoExtract^® Native Membrane Protein Extraction Kit (M-PEK, Cat. No. 444810). With the exception of the collagenase enzyme the set contains all reagents required for efficient dissociation of tissue samples. The goal of the cell isolation procedure is to maximize the yield of viable dissociated cells, so the end-user must select the collagenase appropriate for each tissue with respect to the type of tissue, the species of origin, and the age of the animal. A list of enzymes is provided below in Materials Required but Not Provided.

产品使用声明

预定用途

The Calbiochem^® ProteoExtract^® Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue for the purpose of protein extraction. It is an accessory product for the following kits:

• ProteoExtract^® Subcellular Proteome Extraction Kit (S-PEK, Cat. No. 539790)
• ProteoExtract^® Subcellular Proteome Extraction Kit, Mini (S-PEK Mini, Cat. No. 539791)
• ProteoExtract^® Native Membrane Protein Extraction Kit (M-PEK, Cat. No. 444810).

With the exception of the collagenase enzyme the set contains all reagents required for efficient dissociation of tissue samples. The goal of the cell isolation procedure is to maximize the yield of viable dissociated cells, so the end-user must select the collagenase appropriate for each tissue with respect to the type of tissue, the species of origin, and the age of the animal. A list of enzymes is provided below in Materials Required but Not Provided.

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	+2°C to +8°C
存储条件	Upon arrival store the entire contents of the kit at 4°C. Following reconstitution, all components are stable for a minimum of 2 days at 4°C or for up to 2 months at -20°C. Avoid freeze/thaw cycles of reconstituted solutions.
无需冷冻	No