- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, R, M WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN24 描述 Anti-pan-Shank Antibody, clone N23B/49 Alternate Names - GKAP/SAPAP interacting protein
- SH3 and multiple ankyrin repeat domains 2
- SH3 and multiple ankyrin repeat domains protein 2
- proline-rich synapse associated protein 1
- cortactin SH3 domain-binding protein
- Cortactin-binding protein 1
- Proline-rich synapse-associated protein 1
- cortactin binding protein 1
背景信息 Shank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. 产品信息 格式 Purified 控制 - Rat brain membrane tissue lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue.
Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility.生物信息 免疫原品种 Recombinant protein consisting of SH3/PDZ domain of rat Shank2. 克隆 N23B/49 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 HumanRatMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001004133
基因符号 - Shank2
- SHANK2
- CORTBP1
- ProSAP1
- CTTNBP1
- SPANK-3
- SHANK
- PROSAP1
- CortBP1
纯化方法 Protein G UniProt编号 - Q9QX74
UniProt汇总 FUNCTION: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
SUBUNIT STRUCTURE: Interacts with DNM2 and BAIAP2. Interacts with GRID2 (By similarity). Interacts with CCTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with SLC9A3 and PLCB3. Interacts with DBNL. Interacts with CFTR.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › growth cone. Note: Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.
TISSUE SPECIFICTY: All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
DEVELOPMENTAL STAGE: Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
DOMAIN: The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
SEQUENCE SIMILARIITIES: Belongs to the SHANK family.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.产品使用声明 质量保证 Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MCVD2MAG-77K,MILLIPLEX MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay
下一件merck millipore产品：merck millipore,默克密理博,MABC539,Anti-SFRP2 Antibody, clone 80.8.6, azide free
相关产品
merck millipore,默克密理博,05-936,Anti-ORC2 Antibody, clone 3G6
merck millipore,默克密理博,539720,ProteoExtract Tissue Dissociation Buffer Kit
merck millipore,默克密理博,ABE492,Anti-Histone lysine demethylase NO66 Antibody
merck millipore,默克密理博,681665,Wnt Agonist - CAS 853220-52-7 - Calbiochem
merck millipore,默克密理博,MABC539,Anti-SFRP2 Antibody, clone 80.8.6, azide free
merck millipore,默克密理博,05-591,Anti-Akt/PKB Antibody, PH Domain, clone SKB1
merck millipore,默克密理博,MAB3224,Anti-Basal Cell Cytokeratin Antibody
merck millipore,默克密理博,AP108F,Goat Anti-Guinea Pig IgG Antibody, FITC conjugate
