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merck millipore,默克密理博,MAB3224,Anti-Basal Cell Cytokeratin Antibody
产品名称：Anti-Basal Cell Cytokeratin Antibody
产品型号：MAB3224
Anti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB.
merck millipore,默克密理博,MAB3224,Anti-Basal Cell Cytokeratin Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Gp, Ca, Ch, H, Ht, Po, R, Rb, Qu WB, FC, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3224 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Basal Cell Cytokeratin Antibody Alternate Names - Cytokeratin 5 and others
背景信息 Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. 产品信息 格式 Purified 演示 Purified immunoglobulin. Purified immunoglobulin in buffer with 0.1% sodium azide. 应用 应用 Anti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:100-1:1,000
Immunohistochemistry on frozen tissue sections: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Human cytokeratins. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium. 同种型 IgG1 品种反应性 Guinea PigCanineChickenHumanHamsterPigRatRabbitQuail 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000424.3
Entrez基因汇总 The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. 基因符号 - CK-5
- KRT5
- DDD
- CK5
- K5
- EBS2
- Keratin-5
- KRT5A
- Cytokeratin-5
UniProt编号 - P13647
UniProt汇总 SIZE: 590 amino acids; 62378 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
DISEASE: SwissProt: P13647 # Defects in KRT5 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 131960]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%) and it is characterized by blistering within the basal cell layer. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal-epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema [MIM:609352]. EBS with migratory circinate erythema is milder than the DM-EBS phenotype but involves an unusual migratory circinate erythema with multiple vesicles on the area affected by the erythema. The lesions, which appeared from birth primarily on the hands, feet, and legs but spared the nails, ocular epithelia, and mucosae, healed with brown pigmentation but no scarring. Electron microscopy findings were distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is characterized by additional reticular hyperpigmentation. & Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
SIMILARITY: SwissProt: P13647 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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