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merck millipore,默克密理博,HNPMAG-35K,MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
产品名称:MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
产品型号:HNPMAG-35K
The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Orexin A, Oxytocin, Substance P.
merck millipore,默克密理博,HNPMAG-35K,MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,HNPMAG-35K,MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 Substance P, α-MSH, β-Endorphin, Neurotensin, Orexin A, Oxytocin H Mplex Luminex xMAP 描述 产品目录编号 HNPMAG-35K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay 概述 The MILLIPLEX® MAP Human Neuropeptide Magnetic Bead Panel is to be used for the simultaneous quantification of the following 6 analytes in any combination: α-MSH, β-Endorphin, Neurotensin, Orexin A, Oxytocin, and Substance P. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate, culture supernatant samples, and cerebrospinal fluid (CSF). Serum or plasma samples may also be used, and will need to be extracted.
An important note about this magnetic conversion panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.背景信息 The central nervous system is a complex environment consisting of billions of neurons and glial support cells. These neurons use chemical signals to communicate information. Neuropeptides are neuronal signaling molecules secreted by the central and peripheral nervous systems, influencing a broad spectrum of biological functions including the regulation of metabolism, reproduction, immunity, and behavior.
Understanding the role of neuropeptides in the pathogenesis of devastating neuronal diseases and behavioral disorders is fundamental. While it is understood that accurate measurement of neuropeptides is important, conventional methods, including RIAs and ELISAs, are not able to simultaneously measure multiple neuropeptides with small sample volume.
EMD Millipore’s MILLIPLEX® MAP Human Neuropeptide Magnetic Bead Panel is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples: α-Melanocyte Stimulating Hormone (α-MSH), β-Endorphin, Neurotensin, Orexin A, Oxytocin, and Substance P.产品信息 部件 - Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
- Human Neuropeptide Standard, 1 vial, lyophilized
- Human Neuropeptide Quality Control 1, 1 vial, lyophilized
- Human Neuropeptide Quality Control 2, 1 vial, lyophilized
- Assay Buffer, 2 bottles, 30 mL ea.
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Human Neuropeptide Primary Antibodies, 1 bottle, 3.5 mL
- Human Neuropeptide Detection Antibodies, 1 bottle, 5.5 mL
- Human Neuropeptide Matrix Solution, 1 bottle, 1.5 mL
- Human Neuropeptide Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
- Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
- Mixing Bottle, 1 bottle
检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度(%) - Intra-assay: < 15
- Inter-assay: < 25
板面类型 MAGNETIC Neuroscience 应用 应用 The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Orexin A, Oxytocin, Substance P. 主要应用 - Multiplexing
应用说明 • This is an overnight assay. Note that this is a competitive assay.
• This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well.生物信息 特异性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 品种反应性 Human 可用的分析物 - Substance P
- α-MSH
- β-Endorphin
- Neurotensin
- Orexin A
- Oxytocin
化验面板 Everything you need in a single kit. 交叉反应性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 准确度 - Spike Recovery: 98% - 118%
理化信息 灵敏度 - Refer to kit protocol for sensitivities of individual biomarkers.
- 11 - 479 pg/mL (MinDC+2SD).
标准曲线范围 - Substance P: 7 – 5,000 pg/mL
- α-MSH: 27 – 20,000 pg/mL
- β-Endorphine: 69 – 50,000 pg/mL
- Neurotensin: 41 – 30,000 pg/mL
- Orexin A: 343 – 250,000 pg/mL
- Oxytocin: 55 – 40,000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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