merck millipore,默克密理博,GF101,CD40 Ligand/TRAP Protein, Recombinant human

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,GF101,CD40 Ligand/TRAP Protein, Recombinant human
产品名称：CD40 Ligand/TRAP Protein, Recombinant human
产品型号：GF101
The CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.

merck millipore,默克密理博,GF101,CD40 Ligand/TRAP Protein, Recombinant human

产品介绍

merck millipore,默克密理博,GF101,CD40 Ligand/TRAP Protein, Recombinant human

重要规格表

主要应用	品种	来源	纯度
CULT	Human	Recombinant human	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.

描述
产品目录编号	GF101
品牌系列	Chemicon®
商名	Chemicon
描述	CD40 Ligand/TRAP Protein, Recombinant human
概述	CD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Alternate Names	T cell antigen Gp39 CD154 TRAP TNF-related activation protein

产品信息
演示	Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.

应用
应用	The CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.
主要应用	Cell Culture

生物信息
纯度	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
来源	Recombinant human
品种	Human
特异活性	GF101 is fully biologically active as determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PMBC). It also stimulates IL-8 induction by human PBMC using a concentration of 5-10 ng/mL.
Entrez基因编号	NM_000074.2
Entrez基因汇总	The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
基因符号	T-BAM CD40LG CD154 TRAP gp39 CD40L IMD3 IGM hCD40L TNFSF5 HIGM1 CD40-L
UniProt编号	P29965
UniProt汇总	FUNCTION: SwissProt: P29965 # Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. SIZE: 261 amino acids; 29274 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & CD40 ligand, soluble form: Secreted. TISSUE SPECIFICITY: Specifically expressed on activated CD4+ T- lymphocytes. PTM: The soluble form derives from the membrane form by proteolytic processing. & N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. & Not O-glycosylated. DISEASE: SwissProt: P29965 # Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. SIMILARITY: SwissProt: P29965 ## Belongs to the tumor necrosis factor family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in 10 mM Sodium Phosphate, pH7.5 to a concentration of 0.1-1 mg/mL. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.

储存和货运信息

存储条件

The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in 10 mM Sodium Phosphate, pH7.5 to a concentration of 0.1-1 mg/mL. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.