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品种反应性 主要应用 宿主 格式 抗体类型 Rb, H, R WB, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-445 Replaces 04-240 品牌系列 Upstate
商名 - Upstate
描述 Anti-Kip1 (p27) Antibody 背景信息 p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. 产品信息 格式 Purified 控制 - Positive Control Included: HeLa Nuclear Extract (12-309). Other controls: MCF-7 cell lysate
演示 Protein A Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. 应用 应用 Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB. 主要应用 - Western Blotting
- Immunohistochemistry
生物信息 免疫原品种 An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Kip1. 同种型 IgG 品种反应性 RabbitHumanRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004064
Entrez基因汇总 This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. 基因符号 - KIP1
- CDKN1B
- p27Kip1
- P27KIP1
- MEN1B
- MEN4
- CDKN4
纯化方法 Protein A purfied UniProt编号 - P46527
UniProt汇总 FUNCTION: SwissProt: P46527 # Involved in G1 arrest. May mediate TGF beta-induced G1 arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin NUP50 is required for nuclear import and for degradation of phosphorylated p27Kip1 after nuclear import (By similarity).
SIZE: 198 amino acids; 22073 Da
SUBUNIT: Interacts with NUP50 and with UHMK1. Interacts with COPS5 subunit of COP9 signalosome complex, leading to its subsequent degradation (By similarity). Interacts with SPDYA and is found in a complex with both SPDYA and CDK2.
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. Upon cell cycle progression, mostly cytoplasmic (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
DOMAIN: SwissProt: P46527 A peptide sequence containing only AA 28-79 retains substantial KIP1 cyclin A/CDK2 inhibitory activity.
PTM: Phosphorylation on Ser-10 leads to nuclear export to the cytoplasm and promotes cell cycle progression (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: P46527 # Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
SIMILARITY: Belongs to the CDI family.产品使用声明 质量保证 routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
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