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merck millipore,默克密理博,FCMAB346F,Milli-Mark Anti-NFκB p52-FITC Antibody
产品名称：Milli-Mark Anti-NFκB p52-FITC Antibody
产品型号：FCMAB346F
Milli-Mark Anti-NFκB p52-FITC Antibody detects level of NFκB p52 & has been published & validated for use in FC.
merck millipore,默克密理博,FCMAB346F,Milli-Mark Anti-NFκB p52-FITC Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M FITC Monoclonal Antibody 描述 产品目录编号 FCMAB346F 商名 - Milli-Mark
描述 Milli-Mark® Anti-NFκB p52-FITC Antibody Alternate Names - nuclear factor of kappa light chain gene enhancer in B-cells 2 (p49/p100)
- DNA-binding factor KBF2
- Lymphocyte translocation chromosome 10
- Oncogene Lyt-10
背景信息 The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. 产品信息 格式 FITC 控制 - HeLa cells
演示 Purified mouse monoclonal IgG2a conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 Milli-Mark Anti-NFκB p52-FITC Antibody detects level of NFκB p52 & has been published & validated for use in FC. 主要应用 - Flow Cytometry
生物信息 免疫原品种 GST-fusion protein corresponding to amino acids 1-444 of human NFκB p52 subunit (Schmidt, R., 1991;Bours, V., 1992). 宿主 Mouse 特异性 This antibody recognizes the p52 subunit of NFκB and its p100 precursor. 同种型 IgG2a 品种反应性 Human Species Reactivity Note Tested and passed on Human. Does not cross-react with mouse or rat; other species cross-reactivity is unknown. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_062281
Entrez基因汇总 NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM] . 基因符号 - Lyt10
- NFKB2
- LYT10
- H2TF1
- LYT-10
纯化方法 Protein G Purified UniProt编号 - Q9WTK5
UniProt汇总 FUNCTION: SwissProt: Q00653 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
SIZE: 900 amino acids; 96749 Da
SUBUNIT: Active NF-kappa-B is a heterodimer of an about 52 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
DOMAIN: SwissProt: Q00653 The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation (By similarity). & The glycine-rich region (GRR) appears to be a critical element in the generation of p52.
PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. & Constitutive processing is tightly suppressed by its C- terminal processing inhibitory domain, named PID, which contains the death domain.
DISEASE: SwissProt: Q00653 # A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant. & A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT). & In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.
SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain.产品使用声明 质量保证 Evaluated by flow cytometry using HeLa cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 tests
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