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重要规格表
纯度 ≥95% by SDS-PAGE 描述 概述 Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins. 产品目录编号 565834 品牌系列 Calbiochem®
参考 参考 Hoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859. 产品信息 形式 Lyophilized 配方 Lyophilized from 0.2 µm filtered PBS, BSA. 生物信息 生物活性 Proliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotxin: ≤1 EU/µg 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
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