merck millipore,默克密理博,09-107,Anti-phospho-Raptor (Ser877) Antibody

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merck millipore,默克密理博,09-107,Anti-phospho-Raptor (Ser877) Antibody
产品名称：Anti-phospho-Raptor (Ser877) Antibody
产品型号：09-107
This Anti-phospho-Raptor (Ser877) Antibody is validated for use in WB for the detection of phospho-Raptor (Ser877).

merck millipore,默克密理博,09-107,Anti-phospho-Raptor (Ser877) Antibody

产品介绍

merck millipore,默克密理博,09-107,Anti-phospho-Raptor (Ser877) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Mk, H, Pm	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	09-107
描述	Anti-phospho-Raptor (Ser877) Antibody
Alternate Names	regulatory associated protein of mTOR P150 target of rapamycin (TOR)-scaffold protein p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
背景信息	Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates

产品信息
格式	Affinity Purified
控制	Jurkat cell lysate
演示	Purified rabbit serum in PBS Other with 50% Glycerol.

应用
应用	This Anti-phospho-Raptor (Ser877) Antibody is validated for use in WB for the detection of phospho-Raptor (Ser877).
主要应用	Western Blotting
应用说明	Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Peterson, T., et al. (2009). Cell. 137:873–886.)

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Ser877.
表位	Ser877
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Raptor at Ser877.
品种反应性	MonkeyHumanPrimate
Species Reactivity Note	Proven to react with human. Predicted to react with primate and monkey based on 100% sequence homology. Mouse and rat (90%).
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_065812
Entrez基因汇总	This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
基因符号	Raptor raptor
修改	Phosphorylation
纯化方法	Affinity Purfied
UniProt编号	Q8N122
UniProt汇总	FUNCTION: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions. SIZE: 1335 amino acids; 149038 Da SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. SIMILARITY: Contains 7 WD repeats. MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity.

产品使用声明
质量保证	Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected Raptor on 10 µg of Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.