merck millipore,默克密理博,NA81,Anti-5-Methylcytosine Mouse mAb (162 33 D3)

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merck millipore,默克密理博,NA81,Anti-5-Methylcytosine Mouse mAb (162 33 D3)
产品名称：Anti-5-Methylcytosine Mouse mAb (162 33 D3)
产品型号：NA81
This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

merck millipore,默克密理博,NA81,Anti-5-Methylcytosine Mouse mAb (162 33 D3)

产品介绍

merck millipore,默克密理博,NA81,Anti-5-Methylcytosine Mouse mAb (162 33 D3)

重要规格表

宿主
M

描述
概述	Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells. Specific • Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications • Flow Cytometry • Frozen Sections • Immunoblotting • Immunofluorescence • Paraffin Sections Reliable • Generates reproducible results. • Clone 162 33 D3 has been used in over 50 publications.
产品目录编号	NA81
品牌系列	Calbiochem®
同义词	Anti-5-mc, Anti-5-MeCyd
应用数据	Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.

参考
参考	Ehrlich, M. 2002. Oncogene 21, 5400. (Review) Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review) Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983. Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856. Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114. Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251. De Capoa, A., et al. 1999. FASEB J. 13, 89.

参考

Ehrlich, M. 2002. Oncogene 21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
De Capoa, A., et al. 1999. FASEB J. 13, 89.

产品信息
形式	Liquid
配方	In PBS, pH 7.4.
正控制	NIH3T3 cells
防腐剂	None

应用
应用参考	Paraffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.
主要应用	Enzyme-Linked Immunosorbent Assay Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Paraffin Sections Radioimmunoassay Southwestern Blot

生物信息
免疫原	5-methylcytosine conjugated to ovalbumin
克隆	162 33 D3
宿主	Mouse
同种型	IgG₁
品种	a broad range of species
浓缩标签	Please refer to vial label for lot-specific concentration

储存和货运信息
货运代码	Blue Ice Only
毒性	Standard Handling
储存	-20°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial thaw, aliquot and freeze (-20°C).