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merck millipore,默克密理博,FCIM025161,FlowCellect™ Mouse Th1 Differentiation Tool Kit
产品名称：FlowCellect™ Mouse Th1 Differentiation Tool Kit
产品型号：FCIM025161
The FlowCellect Mouse Th1 Differentiation Tool Kit for Flow Cytometry includes a CD3 Coated Activation Plate, complete Differentiation Media & Activator/Re-Stimulation Reagents.
merck millipore,默克密理博,FCIM025161,FlowCellect™ Mouse Th1 Differentiation Tool Kit
- 产品介绍
描述 产品目录编号 FCIM025161 商名 - FlowCellect
描述 FlowCellect™ Mouse Th1 Differentiation Tool Kit 概述 The FlowCellect Mouse™ Th1 Differentiation Tool Kit includes a CD3 Coated Activation Plate, complete Differentiation Media and Activator/Re-Stimulation Reagents. Detailed assay instructions are included to assist in three culture steps: Activation, Expansion and Re-Stimulation to complete Th1 cell generation in 6 days.
Day 1=Activation: Culture CD4+ cells with growth factors and TCR co-stimulator on an anti-CD3 coated 96-well plate for 3 days.
Day 4=Expansion: Expand activated/developing Th1 cells in a larger tissue culture plate.
Day 6=Re-Stimulation: Differentiated Th1 cells are re-stimulated with mitogens to induce Th1 signature cytokine production on day 6.Alternate Names - Flow Cellect
背景信息 CD4+ T cells play a central role in the function of the immune system. They help B cells produce antibodies, enhance and maintain responses of CD8+ T cells, regulate macrophage function, orchestrate immune responses against a wide variety of pathogenic microorganisms, and regulate immune responses.
Effector T cells are derived from naïve CD4+ progenitor T cells, after maturational complex interactions with antigen-presenting cells in a permissive milieu, including antigenic type and load, and cytokine signaling. Committed CD4+ T cells can differentiate into four major lineages: Th1, Th2, Th17 and regulatory T cells with distinct cytokine products and biological functions.
The primary role of the Th1 subtype is to address intracellular pathogens, by producing IFNgamma (IFNg), interleukin (IL) -2 and tumor necrosis factor alpha/beta to help cellular immunity. The differentiation to the Th1 lineage is promoted through a series of complex signaling events such as IL-12 which is mainly produced by macrophages as well as IFNg which produced by NK, CD8 and Th1 cells. In addition, neutralizing antibody-mediated IL-4 depletion enhances the generation of Th1 cells in vitro, suggesting that IL-4 which is signature cytokine for Th2 lineage, antagonizes Th1 polarization.产品信息 部件 - 5. Re-Stimulation Reagent: (Part No.CS206520) One vial containing 48 μL cocktail of PMA and ionomycin in DMSO
- 1. Mouse CD3 Coated Activation Plate: (Part No. CS206556) One 96-well plate with strips.
- 2. Th1 Activator #1: (Part No. CS206522) One vial containing 33 µL of neutralizing antibodies.
- 3. Differentiation Media: (Part No. CS206524) One vial containing 200 mL of complete Th1 differentiation culture media
- 4. Th1 Activator #2: (Part No.CS206523) One vial containing lyophilized growth factors
应用 应用 The FlowCellect Mouse Th1 Differentiation Tool Kit for Flow Cytometry includes a CD3 Coated Activation Plate, complete Differentiation Media & Activator/Re-Stimulation Reagents. 主要应用 - Flow Cytometry
生物信息 宿主 Mixed Species 品种反应性 Mouse 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 12 Tests / Kit
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