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品种反应性 主要应用 宿主 格式 抗体类型 Mk, Ca, H, M WB, ELISA, FC R Purified Monoclonal Antibody 描述 产品目录编号 MAB3507 Replaces 04-571 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caspase 2 Antibody, clone 11B4 Alternate Names - ICH-1
产品信息 格式 Purified 演示 Liquid with 0.05% Bronidox as a preservative. 应用 应用 Anti-Caspase 2 Antibody, clone 11B4 is an antibody against Caspase 2 for use in ELISA, FC, IP & WB. 主要应用 - Western Blotting
- ELISA
- Flow Cytometry
应用说明 Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.
Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.
EIA: Recommended concentration 0.5-1 μg/ml.
Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Raised against the P19 subunit of recombinant human Caspase-2 protein. 克隆 11B4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 MAB3507 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2). 同种型 IgG2a 品种反应性 MonkeyCanineHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_032983.2
- NM_032982.2
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. 基因符号 - Caspase-2 subunit p12].
- CASP2
- NEDD2
- ICH1
- NEDD-2
- ICH-1L/1S
- CASP-2
- ICH-1L
- EC 3.4.22.55 [Contains: Caspase-2 subunit p18
- Caspase-2 subunit p13
UniProt编号 - P42575
UniProt汇总 FUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
SIZE: 452 amino acids; 50685 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
PTM: The mature protease can process its own propeptide, but not that of other caspases.
SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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