- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,ECM556,QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
产品名称：QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
产品型号：ECM556
The Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells.
merck millipore,默克密理博,ECM556,QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
- 产品介绍
重要规格表
检测方法 Fluorescent 描述 产品目录编号 ECM556 品牌系列 Chemicon®
商名 - Chemicon
- QCM
描述 QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric 概述 Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!
Introduction
Penetration of the subendothelial basement membrane marks a critical turning point in the metastatic process. As proliferating neoplastic cells attempt to escape the primary tumor site, local invasion of the surrounding tissue (interstitial stroma) must occur. Neovascularization is initiated by expression of angiogenic factors (e.g. FGF, VEGF, HGF), providing nutritional requirements and access to the vascular system. Prior to penetrating the blood vessel endothelium and gaining access to the blood stream (intravasation), cancer cells must invade local tissues by degrading ECM components and ultimately, transverse the basement membrane. Once in circulation, these cells can form metastatic colonies at secondary locations, making this membrane a key invasive barrier.
The basement membrane surrounding the blood vessel endothelium is a thin, specialized network of extracellular matrix proteins (ECM) that serves many functions. Comprised of proteins and proteoglycans, such as collagen, laminin, entactin, fibronectin, heparin sulfate and perlecan, this membrane acts as a physical barrier between the epithelium and underlying tissues. It provides cell surface anchorage (via integrins, receptor kinases, and cell surface proteoglycans), induces cellular differentiation, gives architectural support, and limits the migration of normal cells. The ability of tumor cells to degrade the ECM components of the basement membrane and surrounding tissues is directly correlated with metastatic potential. By releasing proteolytic enzymes (e.g. MMP collagenases, plasminogen activators, cathepsins), cancer cells are able to breach the membrane and penetrate the blood vessel wall (1). Collagen, the primary structural element of the basement membrane and tissue scaffolding protein, represents the main deterrent in the migration of tumor cells.
The ability to study cell invasion through a collagen barrier, is of vital importance for developing possible metastatic inhibitors and therapeutics. The new CHEMICON QCM™ 96-well Collagen-based Invasion Assay (ECM556) provides an efficient, in vitro system for quantitative, high-throughput analysis of tumor cell invasion.
In the QCM™ 96-well Collagen-based Invasion Assay (ECM556), invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with a specially formulated Cell Detachment Buffer. The invasive cells are subsequently lysed and detected by the patented CyQuant dye (Molecular Probes) (2-3). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (4).
The CHEMICON QCM™ 96-well Collagen-based Invasion Assay (ECM556) eliminates cell pre-labeling, fixing/staining, swabbing, and manual counting. The 96-well insert and homogenous fluorescence detection format allows for high-throughput, quantitative comparison of multiple samples.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:- QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
- QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
- QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
- QCM™ 96-well Cell Invasion Assay (ECM555)
- 24-well Insert Cell Migration and Invasion Assay Systems
- CytoMatrix™ Cell Adhesion strips (ECM protein coated)
- QuantiMatrix™ ECM protein ELISA kits
Test Principle
The CHEMICON Cell Invasion Assay is performed in a 96-well invasion plate based on the Boyden chamber principle. This plate contains 96 inserts; each insert contains an 8 μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. The collagen layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the polymerized collagen layer and cling to the bottom of the polycarbonate membrane. Invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer and subsequently lysed and detected by CyQuant GR dye.产品信息 部件 - CyQuant GR Dye®1: (Part No. 90132) One vial - 75 mL
- Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90249) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with collagen-coated inserts.
- 96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
- Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
- 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
检测方法 Fluorescent 应用 应用 The Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. 应用说明 The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 96 samples. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.生物信息 品种反应性 Vertebrates 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store kit materials at 2-8°C for up to their expiration date. Do not freeze. 包装信息 数量 1 plate 包装 96 wells
上一件merck millipore产品：merck millipore,默克密理博,ABC340,Anti-GOLPH1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,204001,BX-795 - CAS 702675-74-9 - Calbiochem
相关产品
merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3
merck millipore,默克密理博,AG217,Histamine Receptor 4, control peptide for AB5663P
merck millipore,默克密理博,AB10002,Anti-mouse BID cleavage (59/60) site specific Antibody
merck millipore,默克密理博,ELLUR0200,Luminata Crescendo ELISA HRP Substrate; 200 mL
merck millipore,默克密理博,ABC340,Anti-GOLPH1 Antibody
merck millipore,默克密理博,204001,BX-795 - CAS 702675-74-9 - Calbiochem
merck millipore,默克密理博,MAB045X,Anti-Fluorescein Antibody, clone 5D6.2, Alexa Fluor 488 conjugated
merck millipore,默克密理博,444105,Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)
merck millipore,默克密理博,14-197M,Casein Kinase 2 Protein, active, 250 µg
