merck millipore,默克密理愽
  • merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3

  • merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3

    产品名称：Anti-HDAC2 Antibody, clone 3F3
    产品型号：05-814
    Anti-HDAC2 Antibody, clone 3F3 is a Mouse Monoclonal Antibody for detection of HDAC2 also known as YY1-associated factor 1, histone deacetylase 2 & has been validated in ICC, IP & WB.

    merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3

  • 产品介绍
  • merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Ht, H, B, M, RWB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-814
    Replaces 04-229
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-HDAC2 Antibody, clone 3F3
    Alternate Names
    • transcriptional regulator homolog RPD3
    • YY1-associated factor 1
    • histone deacetylase 2
    背景信息 Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
    产品信息
    格式 Purified
    控制
    • Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
    演示 Purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
    应用
    应用 Anti-HDAC2 Antibody, clone 3F3 is a Mouse Monoclonal Antibody for detection of HDAC2 also known as YY1-associated factor 1, histone deacetylase 2 & has been validated in ICC, IP & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunocytochemistry:
    This antibody has been reported by an independent laboratory to show positive immunostaining for HDAC2 in NIH/3T3 and U2OS cells fixed with paraformaldehyde.

    Immunoprecipitation and HDAC Assay:
    3 μL of a previous lot was used to immunoprecipitate HDAC activity from HeLa nuclear extract, which was then measured using the HDAC Assay Kit (Fluorometric Detection) (Catalog # 17-356). An independent laboratory has also demonstrated positive immunoprecipitation /HDAC assay results using NIH/3T3 cells.
    生物信息
    免疫原品种 KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to amino acids 473-488 of human HDAC2 (Accession # NP_001518) containing a N-terminal cysteine
    克隆 3F3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes HDAC2, MW ~60 kDa.
    同种型 IgG1
    品种反应性 HamsterHumanBovineMouseRat
    Species Reactivity Note Human, mouse, bovine and hamster. Predicted to cross-react with rat based on sequence homology.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_001518
    Entrez基因汇总 This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events.
    基因符号
    • RPD3
    • HDAC2
    • HD2
    • YAF1
    纯化方法 Protein G purfied
    UniProt编号
    • Q92769
    UniProt汇总 FUNCTION: SwissProt: Q92769 # Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1.
    SIZE: 488 amino acids; 55364 Da
    SUBUNIT: Interacts with the non-histone region of H2AFY (By similarity). Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, DNMT1, MINT, HDAC7, HDAC10, HCFC1, NRIP1, MJD2A/JHDM3A, SAP30, SETDB1 and SUV39H1. Interacts with the non-histone region of H2AFY. Interacts with PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with CBFA2T3. Interacts with SAP30L.
    SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Widely expressed; lower levels in brain and lung.
    SIMILARITY: SwissProt: Q92769 ## Belongs to the histone deacetylase family. Type 1 subfamily.
    产品使用声明
    质量保证 Evaluated by western blot on nuclear extracts from HeLa cells.

    Western Blot Analysis:
    0.5-2 μg/mL of this antibody detected HDAC2 in nuclear extracts from HeLa cells.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 200 µg

    merck millipore,默克密理博,05-814,Anti-HDAC2 Antibody, clone 3F3

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