merck millipore,默克密理愽
  • merck millipore,默克密理博,ECM509,QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

  • merck millipore,默克密理博,ECM509,QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

    产品名称：QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
    产品型号：ECM509
    The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration.

    merck millipore,默克密理博,ECM509,QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

  • 产品介绍
  • merck millipore,默克密理博,ECM509,QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

    重要规格表

    主要应用 检测方法
    ACTFluorescent
    描述
    产品目录编号 ECM509
    品牌系列 Chemicon®
    商名
    • Chemicon
    • QCM
    描述 QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
    概述 Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!

    Introduction
    Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

    The CHEMICON® QCM™ 24-well Cell Migration Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples.

    In the CHEMICON® QCM™ 24-well Migration Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQUANTâ GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3).

    The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

    The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.



    In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

    · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

    · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

    · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

    · QCM™ 96-well Cell Invasion Assay (ECM555)

    · QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)

    · 24-well Insert Cell Migration and Invasion Assay Systems

    · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

    · QuantiMatrix™ ECM protein ELISA kits

    Test Principle

    The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.
    产品信息
    部件
    • Forceps: (Part No. 10203) One each.
    • Sterile 24-well Cell Migration Plate Assembly: (Part No. 90333) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
    • Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
    • 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
    • CyQUANTâ GR Dye1: (Part No. 90132) One vial - 75 μL
    检测方法 Fluorescent
    应用
    应用 The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration.
    主要应用
    • Activity Assay
    应用说明 The CHEMICON® QCM™ 24-well Migration Assay is ideal for the study of chemotaxis cell migration. Each Chemicon Cell Migration Assay Kit contains sufficient reagents for the evaluation of 24 samples. The quantitative nature of this assay is especially useful for screening of pharmacological agents.

    The CHEMICON® QCM™ 24-well Migration Assay is intended for research use only; not for diagnostic applications.
    生物信息
    品种反应性 All
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store kit materials at 2-8°C for up to their expiration date. Do not freeze.
    包装信息
    数量 1 plate
    包装 24 wells

    merck millipore,默克密理博,ECM509,QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

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