merck millipore,默克密理博,ECM310,QuantiMatrix Human Laminin ELISA

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merck millipore,默克密理博,ECM310,QuantiMatrix Human Laminin ELISA
产品名称：QuantiMatrix Human Laminin ELISA
产品型号：ECM310

merck millipore,默克密理博,ECM310,QuantiMatrix Human Laminin ELISA

产品介绍

merck millipore,默克密理博,ECM310,QuantiMatrix Human Laminin ELISA

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
Laminin	H	ELISA	Chromogenic

描述
产品目录编号	ECM310
品牌系列	Chemicon®
商名	Chemicon Quantimatrix
描述	QuantiMatrix Human Laminin ELISA
概述	The QuantiMatrix™ ELISA kit for the determination of soluble human laminin provides sensitivity down to 30-70 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human laminin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human laminin. The polyclonal antibody binds to laminin in the standards and samples. The mixture is then transferred to the human laminin-coated plate. Free rabbit anti-human laminin binds to the laminin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-laminin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the concentration of laminin in the original sample. A standard curve is constructed and sample values are interpolated. ECM310 QuantiMatrix Human Laminin ELISA kit is prepared with antibody made against pepsinized human placental laminin and the Control Standard in the kit is also a pepsinized placental laminin preparation. The most common laminin chains expressed in human placenta are generally the alpha 1, alpha 2 and beta 1, beta 2 chains of Laminin. Laminin 5 (alpha3beta3gamma2) is also present in placenta but we have not tested the ECM310 specifically with Laminin 5. It is possible that the kit would detect laminin 5 but we suspect that the signal for laminin 5 might be very weak. The strongest signals would probably be expressed with laminins 1 through 4.
背景信息	The determination of levels of extracellular matrix molecules is becoming more important to understanding alterations in matrix function during development and tumorigenesis. Questions of matrix gene regulation and function require a sensitive means of determining the levels of protein expression by cells.

产品信息
部件	Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to 1 L of deionized water as described in step 1 of protocol on page 2. QuantiMatrixTM Laminin Coated Strips (Part No. 60116) - 1 x 96-well strip plate Human Laminin Standard (Part No. 60117) - 1 lyophilized bottle; reconstitute as indicated on label Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. CS216103) - 1 bottle; ready to use Rabbit anti-Human Laminin (Part No. 60118) - 1 bottle polyclonal antibody; ready to use TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution)
检测方法	Chromogenic

产品信息

部件

Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to 1 L of deionized water as described in step 1 of protocol on page 2.
QuantiMatrixTM Laminin Coated Strips (Part No. 60116) - 1 x 96-well strip plate
Human Laminin Standard (Part No. 60117) - 1 lyophilized bottle; reconstitute as indicated on label
Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal
Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. CS216103) - 1 bottle; ready to use
Rabbit anti-Human Laminin (Part No. 60118) - 1 bottle polyclonal antibody; ready to use
TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use
Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution)

检测方法

Chromogenic

应用
主要应用	ELISA

生物信息
品种反应性	Human
可用的分析物	Laminin
Entrez基因编号	NM_002291.1
Entrez基因汇总	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
基因符号	CLM LAMB1 MGC142015
UniProt编号	P07942
UniProt汇总	FUNCTION: SwissProt: P07942 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SIZE: 1786 amino acids; 198066 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), laminin-2 (merosin), and laminin-6 (K-laminin). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. DOMAIN: SwissProt: P07942 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular. SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store the kit and all components at 2-8°C. PRECAUTIONS: Human Laminin was purified from human tissue from donors tested negative for HBsAg, HIV and antibody to HIV. However, all samples and materials should be handled carefully and in accordance with good laboratory practices. The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data. Do not mix or substitute reagents from different kit lots.

储存和货运信息

存储条件

Store the kit and all components at 2-8°C.

PRECAUTIONS: Human Laminin was purified from human tissue from donors tested negative for HBsAg, HIV and antibody to HIV. However, all samples and materials should be handled carefully and in accordance with good laboratory practices.

The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data.

Do not mix or substitute reagents from different kit lots.