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宿主 M 描述 概述 Recognizes the ~60 kDa GBA protein in MCF-7 and HeLa cells and human breast cancer tissue. 产品目录编号 AP1140 品牌系列 Calbiochem®
同义词 Anti-Glucosidase β, Acid 应用数据 
Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence.
Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence.
Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB).参考 参考 Campeau, P.M., et al. 2009. Blood 114, 3181. 产品信息 形式 Liquid 配方 In PBS, pH 7.2. 负控制 293T 正控制 MCF-7 cells, HeLa cells, Human breast cancer tissue 防腐剂 None 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
生物信息 免疫原 A recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein 克隆 2E2 宿主 Mouse 同种型 IgG2a 品种 human 储存和货运信息 货运代码 Blue Ice Only
毒性 Regulatory Review
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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