merck millipore,默克密理愽
  • merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

  • merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

    产品名称：Millicoat™ Human Fibronectin Coated Strips (96-Wells)
    产品型号：ECM101
    Millicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience & flexibility in designing assays.

    merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

  • 产品介绍
  • merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

    重要规格表

    主要应用 检测方法
    ACTChromogenic
    描述
    产品目录编号 ECM101
    品牌系列 Chemicon®
    商名
    • Chemicon
    • Millicoat
    描述 Millicoat™ Human Fibronectin Coated Strips (96-Wells)
    概述 Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with Human Fibronectin. Row H of each strip is coated with BSA which serves as a negative assay control. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
    Alternate Names
    • Formerly <br />under the CytoMatrix<sup>TM</sup> brand name.
    产品信息
    检测方法 Chromogenic
    应用
    应用 Millicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience & flexibility in designing assays.
    主要应用
    • Activity Assay
    应用说明 PROCEDURE:

    NOTE: Optimal assay timing and performance may vary for different cell lines but generally can be obtained using subconfluent cell cultures in the assay described below. Subconfluent cultures can be achieved by splitting cells 1 to 2 days prior to performing the assay.

    1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydrated strips.

    2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.

    3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 2-3 times with PBS containing Ca2+/Mg2+ (200 mL/well).

    4. Add 100 mL of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).

    5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO4, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.

    6. Determine the absorbance at 540 - 570 nm on a microplate reader.
    生物信息
    品种反应性 Human
    Entrez基因编号
    • NM_212482.1
    • NM_002026.2
    • NM_054034.2
    • NM_212474.1
    • NM_212475.1
    • NM_212476.1
    • NM_212478.1
    Entrez基因汇总 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
    基因符号
    • FINC
    • FN1
    • DKFZp686O13149
    • DKFZp686H0342
    • DKFZp686I1370
    • MSF
    • FN
    • CIG
    • DKFZp686F10164
    • LETS
    UniProt编号
    • P02751
    UniProt汇总 FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
    SIZE: 2386 amino acids; 262607 Da
    SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
    SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
    TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    PTM: Sulfated.
    SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 May be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
    包装信息
    数量 1 plate
    包装 96 wells

    merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

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