merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal
产品名称：Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal
产品型号：04-210
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect c-Jun (C-term) using this Anti-c-Jun (C-term) More>>

merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-210
品牌系列	Upstate
商名	Upstate
描述	Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal
Alternate Names	v-jun sarcoma virus 17 oncogene homolog (avian) Activator protein 1 Jun activation domain binding protein Proto-oncogene c-jun V-jun avian sarcoma virus 17 oncogene homolog enhancer-binding protein AP1 jun oncogene v-jun sarcoma virus 17 oncogene homolog
背景信息	Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.

产品信息
格式	Culture Supernatant
控制	HEK293 whole cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect c-Jun (C-term) using this Anti-c-Jun (C-term) Antibody, clone EP693Y, Rabbit validated for use in IP, WB, IC, IH(P).
主要应用	Immunocytochemistry Western Blotting
应用说明	IImmunoprecipitation: 1:50 dilution was used in immunoprecipitation of c-Jun. Immunohistochemistry Analysis: A 1:100-250 dilution of anti-c-Jun, (C-term) EP693Y was used in IHC Immunohistochemical analysis of paraffin-embedded human colon carcinoma. Immunocytochemistry Analysis:

生物信息
免疫原品种	Synthetic peptide directed against the C-terminus of human c-Jun.
表位	C-terminus
克隆	EP693Y
宿主	Rabbit
特异性	This antibody recognizes c-Jun at its C-Terminus.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Not expected to react with mouse and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002228
Entrez基因汇总	This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq].
基因符号	p39 AP-1 AP1 OTTHUMP00000010036 c-Jun
非反应性品种	Rat Mouse
纯化方法	Unpurified
UniProt编号	P05412
UniProt汇总	FUNCTION: SwissProt: P05412 # Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. SIZE: 331 amino acids; 35676 Da SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC. SIMILARITY: SwissProt: P05412 ## Belongs to the bZIP family. Jun subfamily. & Contains 1 bZIP domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HEK293 whole cell lysate. Western Blot Analysis: A 1:5000 dilution of this lot was used to detect c-Jun HEK293 whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.