merck millipore,默克密理愽
  • merck millipore,默克密理博,04-1540,Anti-PICH Antibody, clone 142-26-3

  • merck millipore,默克密理博,04-1540,Anti-PICH Antibody, clone 142-26-3

    产品名称：Anti-PICH Antibody, clone 142-26-3
    产品型号：04-1540
    Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent  More>>

    merck millipore,默克密理博,04-1540,Anti-PICH Antibody, clone 142-26-3

  • 产品介绍
  • merck millipore,默克密理博,04-1540,Anti-PICH Antibody, clone 142-26-3

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HICC, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-1540
    描述 Anti-PICH Antibody, clone 142-26-3
    Alternate Names
    • DNA excision repair protein ERCC-6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
    • excision repair cross-complementing rodent repair deficiency, complementation group 6-like
    • excision repair cross-complementing rodent repair deficiency complementation group 6 - like
    • Tumor antigen BJ-HCC-15
    • PLK1-interacting checkpoint helicase
    • ATP-dependent helicase ERCC6-like
    • excision repair protein ERCC6-like
    • SNF2/RAD54 family protein
    背景信息 Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.
    产品信息
    格式 Purified
    控制
    • HeLa-S3 cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.

    Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
    生物信息
    免疫原品种 Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
    表位 Unknown
    克隆 142-26-3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes Plk1-interacting checkpoint helicase.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_060139
    基因符号
    • MGC131695
    • PICH
    • ERCC6L
    • FLJ20105
    纯化方法 Protein G purfied
    UniProt编号
    • Q2NKX8
    UniProt汇总 FUNCTION: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.

    SUBUNIT STRUCTURE; Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization.

    SUBCELLULAR LOCATION: Centromere. Kinetochore. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.

    PTM: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

    SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

    Contains 1 helicase ATP-binding domain.

    Contains 1 helicase C-terminal domain.

    Contains 2 TPR repeats.

    SEQUENCE CAUTION: The sequence AAM82750.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.

    The sequence BAA90952.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa-S3 cell lysate.

    Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,04-1540,Anti-PICH Antibody, clone 142-26-3

上一件merck millipore产品：merck millipore,默克密理博,217386,Carboxy-PTIO, Sodium Salt - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,MAB2096,Anti-Sialyl Lewis X Antibody, clone KM93


相关产品

merck millipore,默克密理博,AB9420,Anti-Corticotropin-Releasing Factor Receptor2 Antibody merck millipore,默克密理博,AB9420,Anti-Corticotropin-Releasing Factor Receptor2 Antibody

merck millipore,默克密理博,203189,Biotin-X-NHS, Water-Soluble merck millipore,默克密理博,203189,Biotin-X-NHS, Water-Soluble

merck millipore,默克密理博,SF-858,miR-370; Human, Cyanine 3 RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-858,miR-370; Human, Cyanine 3 RNA Detection Probe,SmartFlare

merck millipore,默克密理博,476496,Protein Lysine Methyltransferase Inhibitor, UNC0321 - Calbiochem merck millipore,默克密理博,476496,Protein Lysine Methyltransferase Inhibitor, UNC0321 - Calbiochem

merck millipore,默克密理博,217386,Carboxy-PTIO, Sodium Salt - Calbiochem merck millipore,默克密理博,217386,Carboxy-PTIO, Sodium Salt - Calbiochem

merck millipore,默克密理博,MAB2096,Anti-Sialyl Lewis X Antibody, clone KM93 merck millipore,默克密理博,MAB2096,Anti-Sialyl Lewis X Antibody, clone KM93

merck millipore,默克密理博,MAB5350,Anti-Choline Acetyltransferase Antibody, clone 28C4 merck millipore,默克密理博,MAB5350,Anti-Choline Acetyltransferase Antibody, clone 28C4

merck millipore,默克密理博,16-187,Anti-dimethyl-Histone H3 (Lys9) Antibody, biotin conjugate merck millipore,默克密理博,16-187,Anti-dimethyl-Histone H3 (Lys9) Antibody, biotin conjugate

merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells) merck millipore,默克密理博,ECM101,Millicoat™ Human Fibronectin Coated Strips (96-Wells)

merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal merck millipore,默克密理博,04-210,Anti-c-Jun (CT) Antibody, clone EP693Y, rabbit monoclonal


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部