merck millipore,默克密理愽
  • merck millipore,默克密理博,14-751,Abl (Q252H) Protein, active, 10 µg

  • merck millipore,默克密理博,14-751,Abl (Q252H) Protein, active, 10 µg

    产品名称：Abl (Q252H) Protein, active, 10 µg
    产品型号：14-751
    Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. For use in Kinase Assays.

    merck millipore,默克密理博,14-751,Abl (Q252H) Protein, active, 10 µg

  • 产品介绍
  • merck millipore,默克密理博,14-751,Abl (Q252H) Protein, active, 10 µg

    描述
    产品目录编号 14-751
    品牌系列 Upstate
    商名
    • Upstate
    描述 Abl (Q252H) Protein, active, 10 µg
    概述 N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
    产品信息
    演示 10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 150 mM NaCl, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
    应用
    应用 Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. For use in Kinase Assays.
    主要应用
    • Kinase Assay
    生物信息
    纯度 Purity 51% by SDS-PAGE and Coomassie staining.
    来源 Expressed by baculovirus in Sf21 insect cells.
    品种 Human
    特异活性 1089 U/mg, where one unit of Abl (Q252H) activity is defined as 1 nmol phosphate incorporated into 50 μM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100 μM.
    Entrez基因编号
    • NM_007313.2
    • NM_005157.3
    Entrez基因汇总 The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.
    基因符号
    • ABL
    • ABL1
    • JTK7
    • p150
    • bcr/abl
    • v-abl
    • c-ABL
    蛋白靶 Abl
    纯化方法 Ni2+/NTA-agarose
    靶子系列 TK
    UniProt编号
    • P00519
    UniProt汇总 FUNCTION: SwissProt: P00519 # Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.
    COFACTOR: Magnesium or manganese.
    SIZE: 1130 amino acids; 122873 Da
    SUBUNIT: Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16 (By similarity). Interacts with INPPL1/SHIP2.
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
    TISSUE SPECIFICITY: Widely expressed.
    PTM: Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation. Isoform IB is myristoylated on Gly-2.
    DISEASE: SwissProt: P00519 # A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    SIMILARITY: SwissProt: P00519 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
    产品使用声明
    质量保证 Routinely evaluated by phosphorylation of 50 μM Abltide. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active Abl (Q252H).
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
    Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
    包装信息
    数量 10 µg

    merck millipore,默克密理博,14-751,Abl (Q252H) Protein, active, 10 µg

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