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merck millipore,默克密理博,CYT296,C-Reactive Protein ELISA Kit, canine
产品名称:C-Reactive Protein ELISA Kit, canine
产品型号:CYT296
The canine C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures canine CRP.
merck millipore,默克密理博,CYT296,C-Reactive Protein ELISA Kit, canine
- 产品介绍
重要规格表
可用的分析物 品种反应性 主要应用 C-Reactive Protein Ca ELISA 描述 产品目录编号 CYT296 品牌系列 Chemicon®
商名 - Chemicon
描述 C-Reactive Protein ELISA Kit, canine 概述 Introduction
The canine C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures canine CRP.
Test Principle
With the canine CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-canine CRP antibody. After a thorough wash, polyclonal anti-canine CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.Alternate Names - CRP
产品信息 部件 - Substrate (TMB) Solution, 12 mL, Protect from light. Non-carcinogenic. (Catalog No. CYT296d)
- Stop Solution (diluted phosphoric acid), 12 mL (Catalog No. CYT296e)
- Wash Buffer, 1 packet (PBS with 0.05% Tween 20, pH 7.4 when reconstituted to 1 liter of distilled water) (Catalog Number CYT296f)
- Microwell Strips (96 wells), coated with Anti-canine CRP Antibody, vacuum sealed (Catalog No. CYT296a)
- Conjugate Concentrate (100x) (Anti-canine CRP Antibody, Horseradish Peroxidase Conjugate), 0.13 mL protect from light (Catalog No. CYT296b)
- Canine CRP Standard (10x), 0.25 mL, contains proclin 150® as a preservative (Catalog No. CYT296c)
应用 应用 The canine C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures canine CRP. 主要应用 - ELISA
应用说明 The canine C-reactive protein (CRP) kit is designed to measure the amount of canine CRP in serum or plasma. There are enough reagents included in this kit for one 96-well immuno-assay plate. We recommend running duplicate wells for samples and standards.
For Research Use Only; Not for use in diagnostic procedures.生物信息 品种反应性 Canine 可用的分析物 - C-Reactive Protein
理化信息 灵敏度 - 3.6 μg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date.
Precautions
· Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary.
· HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use.
· Wear gloves and laboratory coats when handling the kit.
· The materials must not be pipetted by mouth.
· Do not drink, eat or smoke in the areas where the kit is being handled.
· Reagents with different lot numbers should not be mixed.包装信息 数量 1 plate 包装 96 wells
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