- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 CBL1502 Replaces 04-1102 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CD45 Antibody, clone MRC OX-1 Alternate Names - LCA
产品信息 格式 Purified 演示 The monoclonal is presented as 0.5mg at 1mg/mL in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application. 应用 应用 This Anti-CD45 Antibody, clone MRC OX-1 is validated for use in FC, IH for the detection of CD45. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Suitable for flow cytometry
Suitable for use on frozen sections
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Rat thymocyte membrane glycoproteins 克隆 MRC OX-1 宿主 Mouse 特异性 The antibody recognises a monomorphic determinant of the rat leucocyte common antigen (LCA, CD45). The antigen recognised is a heavily glycosylated membrane gylcoprotein of M.W. 170kD on thymocytes but M.W. 170- 220kD on other leucocytes (2,3). It is a useful marker for leucocytes as it has not been detected on any other cell type.
FUSION PARTNER: Spleen cells from BALB/c mice were fused with the cells of the NS1 mouse myeloma cell line.同种型 IgG1 品种反应性 Rat 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_080923.2
- NM_002838.3
- NM_080921.2
- NM_080922.2
Entrez基因汇总 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. 基因符号 - EC 3.1.3.48
- PTPRC
- L-CA
- LCA
- CD45
- LY5
- T200
- glycoprotein
- B220
- GP180
UniProt编号 - P08575
UniProt汇总 FUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
SIZE: 1304 amino acids; 147254 Da
SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. 包装信息 数量 500 µg
上一件merck millipore产品：merck millipore,默克密理博,MABN1045,Anti-ACBD3 Antibody, clone EPR8623, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,444274,MMP-2/MMP-9 Inhibitor IV - CAS 292605-14-2 - Calbiochem
相关产品
merck millipore,默克密理博,AB1530,Anti-Peripherin Antibody
merck millipore,默克密理博,MAB2253,Anti-Integrin β1 Antibody, clone 6S6
merck millipore,默克密理博,371720,Anti-Giα-1-Subunit, Internal (159-168) Rabbit pAb
merck millipore,默克密理博,MABF198,Anti-MDA-5 Antibody, clone EPR6743, rabbit monoclonal
merck millipore,默克密理博,MABN1045,Anti-ACBD3 Antibody, clone EPR8623, rabbit monoclonal
merck millipore,默克密理博,444274,MMP-2/MMP-9 Inhibitor IV - CAS 292605-14-2 - Calbiochem
merck millipore,默克密理博,AB15014,Anti-Glycine Receptor α3 subunit Antibody
merck millipore,默克密理博,AB5460-50UL,Anti-Acid Sensitive Ion Channel 2 Antibody, neuronal
merck millipore,默克密理博,SF-1273,UCHL1; Human, Cyanine 5 RNA Detection Probe,SmartFlare